Thyroid History

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    1996 ASPECTS OF A COMMON MISSED DIAGNOSIS: THYROID DYSFUNCTION AND MANAGEMENT Durrant-Peatfield, B.J Journal of Nutritional & Environmental Medicine, Dec96, Vol. 6 Issue 4, p371

    The all too common misdiagnosis of hypothyroidism is the result of poor clinical appraisal and undue reliance on flawed blood tests. The condition is simply and satisfactorily treated by replacement therapy and a plea is made for greater awareness among clinicians.

    INTRODUCTION

    The syndrome which results from a deficient output of thyroid hormone or deficient utilization is at once extraordinarily common and as commonly misdiagnosed [1]. Why it is that the diagnosis of such a frequently seen illness is so often missed is the primary purpose of this survey and attention will be given to the failure of correct management of an easily and rewardingly treatable illness. The term 'myxoedema' was used by Ord [2] (in 1878) when he found atrophy and fibrosis of the thyroid in association with the presence of a water-containing mucopolysaccharide throughout the tissues.
    ....

    HYPOTHYROIDISM: THE UNSUSPECTED ILLNESS

    ....The thyroid is easily damaged by many disease processes and is five times more likely to be damaged in women than men; autoimmune diseases in particular may affect its integrity. Environmental hazards are having an increasing affect on thyroid activity in the present day. Placing on one side carcinomatous change, there are two pathological changes with which we must be concerned.
    First, overactivity of the thyroid, properly called hyperthyroidism or Grave's disease. This is much less common than underactivity and its incidence is largely unchanged at present.
    ....
    Barnes [6] estimated that up to one-third of people approaching mid-life could be affected, though not all require treatment. The term 'myxoedema' should probably be reserved for those patients with little or no thyroid activity. Hypothyroidism may occur in any degree, from a few per cent down on nominal to true myxoedema. Probably anything more than a 15% loss will produce symptoms, which will vary widely from patient to patient leading to diagnostic confusion, which regrettably leads to a diagnosis being missed more often than it is made.
    The causes of hypothyroidism may be summarized as follows:
    (1) primary or secondary failure of hormone production;
    (2) receptor uptake deficiency;
    (3) receptor resistance;
    (4) conversion failure of T4 to T3.

    Failure of Thyroid Hormone Production

    This may be conveniently classified into the following:
    (1) iodine deficiency;
    (2) genetic;
    (3) secondary to antibody damage;
    (4) pituitary failure (i.e. failure of TSH production), secondary to antibody damage;
    (5) environmental toxins or insufficiencies;
    (6) surgery, i.e. a major operation--hysterectomy, cholecystectomy or tonsillectomy (damage to blood supply);
    (7) major trauma;
    (8) infectious mononucleosis [7];
    (9) treatment of previous thyroid overactivity.

    Receptor Uptake Deficiency

    This may be primary, owing to failure at the site of the portal of entry into the cell, or secondary, due to a lowered metabolic activity at the receptor site, resulting from long exposure to hypothyroidism.

    Receptor Resistance

    Less often seen, this may prove a puzzling diagnostic problem, since all thyroid levels may be normal, yet the symptoms are perfectly clear. It may be associated with partial adrenal insufficiency.

    Conversion Failure of T4 to T3

    The 5'-diiodase enzyme system may be damaged (often as the result of prolonged hypothyroidism) so that, with normal levels of T4, the T3 levels are low. This is again seen in adrenal insufficiency and other conditions as in iodine, iron and selenium deficiency.

    SYMPTOMS AND SIGNS OF HYPOTHYROIDISM

    One of the diagnostic difficulties is the fact that the symptoms are legion; they vary from patient to patient and may be put down to any number of other causes [8]. Yet taken together, with a high index of suspicion on the part of the physician, there should really be no problem.
    ....

    DIAGNOSIS

    This should be a clinical one and should already be clear to the clinician. Further useful evidence is the basal body temperature, first described by Broda Barnes [6, 12].
    ....
    In many cases, a careful history, physical examination and the basal body temperature should be quite enough to establish the diagnosis.
    Clinicians rely unreasonably on the blood pathology, estimating the serum T4, the THUT, the free T3 and TSH. The reference ranges are impossibly too broad [13] and a number of factors may vitiate the results. A reduced blood volume concentrates the blood levels, there is slow clearance from the blood and thyroid hormone varies in a dynamic way. Receptor failure may mean that the blood level bears little relation to the intracellular T[sub 3]. For these and other reasons the blood chemistry must be used with great care and not used only diagnostically. Undue reliance on the chemistry has meant that, in the present writer's experience, in nine cases out of ten, where the symptoms and signs are perfectly clear, the diagnosis is missed. This is regrettable and sad, since hypothyroidism is so easily and successfully treated and the main purpose of this paper is to draw clinicians' attention to this common and unfortunate diagnostic omission. If the history, examination and basal body temperature add up, the diagnosis may be properly and successfully made. A trial of treatment will soon confirm the diagnosis.

    TREATMENT

    The satisfactory management of the hypothyroid state is extraordinarily rewarding for both the clinician and patient. It is not difficult and does not require repeated blood estimations as is commonly believed and, again contrary to common belief, has almost no hazards. The aim is to restore tissue levels to normal, correcting all the functional deficits in the working of all the organs of the body. In this, clinicians should take as their primary purpose the treatment of the patient and not the disease and, most especially, not the blood test.
    ....
    In its simplest classical form, replacement therapy employs 50 mug thyroxine tablets. Treatment may be initiated by the use of 25-50 mug daily. This dose, over a period of 2 or 3 months, may be slowly increased every 2, 3 or 4 weeks according to response. It is most important to realize that the response is slow and requires 2 or 3 weeks to evaluate.
    ....
    Replacement is for life, albeit with dosage variations. It can be discontinued at any time, of course; the improvement will then slowly wear off over weeks and provide any further conviction the clinician or patient needs.
    While this simple management is satisfactory for simple, uncomplicated hypothyroidism, of not too long duration, many patients will not fully respond or, worse, report unpleasant palpitations or flushes or headaches, sometimes within days, thus casting doubt on the diagnosis. It is of great importance that the reason these problems occur should now be considered. First, a poor response may simply be due to insufficiency in the dose. If the dosage regime outlined above is adhered to, there should be a positive response within 4 weeks. If not, clearly there is a failure of uptake and/or utilization. Similarly, apparently toxic symptoms may be the result of an initial overdose, but may equally well be due to the above, causing an effective overdose owing to non-utilization. Assuming the vitamin, mineral and trace element levels are acceptable, the problem is due to either receptor deficiency, adrenal insufficiency or conversion deficiency of T4 to T3 by a weak 5'diiodase enzyme,
    ....
    A low adrenal reserve is a likely problem with marked and long-standing hypothyroidism and the poor response or toxic symptoms may be completely prevented by the use of a low, physiological dose of hydrocortisone [ 17]. A dose of 5 mg of hydrocortone qds is usually sufficient. However, 2.5-5 mg of prednisolone, or deltacortril, daily is often a preferred alternative. This should be continued for 2 months or so and then stopped, since the general improvement the thyroid hormone will have provided will normalize the adrenal function and supplementary glucocorticoid is not then required. It is not usually necessary to stage the reduction; at most halve the dose for a week, then stop. This regime will provide a speedy, safe and smooth response to thyroid hormone and should be used whenever a poor adrenal reserve is suspected. A low blood pressure--which does not rise on the patient standing--a history of collapses and prostration and arthralgia should raise suspicion, and prolonged undiagnosed hypothyroidism will most frequently be paralleled by adrenal insufficiency. The most satisfactory regime is to initiate glucocorticoid for a week prior to the use of thyroid hormone; there may be a general improvement at once since receptor uptake may rapidly be increased. If, in spite of these precautions, problems of response are still worrying, T4 to T3 conversion deficiency may be suspected. Five or ten per cent of cases may suffer this problem.
    ....
    The thyroid produces two thyroid hormones, T4 and T3 in a 5:1 ratio, but a third is suspected [18]. Since replacement should logically be as close to the natural as possible, there is a strong case for the use of T4 and T3 in combination. While most authorities consider this to be an unnecessary complication, it is a rational approach and consideration should be given to it;
    ,,,,
    Two further matters require attention. One is the fear of precipitating cardiovascular collapse, which worries many clinicians. It cannot be too strongly emphasized that the risk to the healthy heart, using the correct dosage regimes outlined above, is non-existent. It is overworking a heart damaged by disease and coronary artery insufficiencies or in failure that problems arise.
    ....
    The detection and treatment of hypothyroidism in children is desperately important but sadly often missed. Cretinism is rare and well recognized, but lesser degrees of hypothyroidism may blight a life if untreated. The child may be sleepy, with an overweight tendency and may develop slowly and below its percentile. Often, however, the child may be of poor stature, with a lumbar lordosis and protuberant belly, underweight and sickly, with a tendency to acquire any infection that is going and, surprisingly, hyperkinetic.
    ....
    Hypothyroidism i.n women is of special importance and some elaboration is necessary. The first symptoms may occur at the menarche; menses may occur unusually early--age 10 or 11 years--or unusually late--16 or 17 years. They may be irregular and unusually heavy or light, often with more dysmenorrhoea and clots than is usual. As the years pass, these symptoms may worsen and severe PMT is frequently found [26]. Fertility is downgraded and pregnancies may be associated with all sorts of problems, including inexplicable miscarriages. The production of extra T3 by the foetus which may occur results in unusually large babies; if diabetes of pregnancy can be excluded, a birth weight over 8 lb 8 oz (4 kg) should raise suspicion.
    ....
    The diagnosis should be essentially clinical and a trial of treatment instituted. Thoughtful management of the trial eliminates any risk of inappropriate treatment and in the writer's view is an entirely acceptable method of confirming the diagnosis without any risk to the patient. Many people suffer needlessly and have their lives blighted by the failure to treat this common and easily diagnosable illness.

    REFERENCES

    [1] Jackson AS. Hypothyroidism. JAMA 1957; 163: 2.
    [2] Ord W. On myxoedema. Trans Med Chiurg Soc 1878; 6061: 57.
    [3] Murray GR. Notes on the treatment of myxoedema by hypodermic injection of extract of thyroid gland of sheep. BMJ 1891; ii: 796.
    [4] Murray GR. Life history of first case of myxoedema treated by thyroid extract. BMJ 1920; ii: 359.
    [5] Hertzog E. Treatment of myxoedema. Int Clin Week 1915; 14 April.
    [6] Barnes B. The Unsuspected Illness. London: Harper & Row, 1976.
    [7] Demitract et al. Evidence for impaired activation of hypothalamic axis in chronic fatigue. J Clin Endocrin 1991; 73.
    [8] Editorial. Puzzling cases and low thyroid function. BMJ 1970.
    [9] Whybrow PC, Prange AJ, Treadway CP. Mental changes accompanying thyroid dysfunction. Arch Gen Psychiatr 1969; 20: 48-63.
    [10] Furunculosis: aetiology & treatment. J Clin Endocrinol 1943; 3.
    [11] Chaery WC. Tendon reflexes in myxoedema. JAMA 1924; 82: 2013-16.
    [12] Barnes B. Basal temperature verses basal metabolism. JAMA 1942; 119: 1072.
    [13] Barnes, Barnes. The Fallacy of Thyroid Function Tests. Riddle of Heart Attacks. Robinson Press, 1976.
    [14] Jeffries WM. Safe Uses of Cortisone. Charles C. Thomas, 1981.
    [15] Present status of ACTH, cortisone and related steroids. New Engl J Med 1955; 253: 441.
    [16] Jeffries WM. Cortisol and Immunity. Medical Hypotheses. Longman, 1991.
    [17] Jeffries WM. Low dosage glucocorticoid therapy. Arch Int Med 1967; 119: 265.
    [18] Barnes B. Is there a third thyroid hormone? J IAPM 1982.
    [19] Barnes B. Eighteen year follow up on thyroid therapy in prophylaxis & treatment of coronary heart disease. Fed Proc 1969; 28516.
    [20] Buslenio PA et al. Pre clinical hypothyroidism: a risk factor in coronary heart disease. Lancet 1971; 1: 203-4.
    [21] Barnes B. Prophylaxis of ischaemic heart disease by thyroid therapy. Lancet 1958; 11: 149.
    [22] Fishbeng. Atherosclerosis in thyroid deficiency. JAMA 1924; 82: 463.
    [23] Modesc, Danoski. Alterations in cholesterol and lipoprotein in euthyroid adults. Circulation 1958; 18: 761.
    [24] Barnes J. Clin Exp Pharmacol Physiol 1975; 167 (suppl. 2): 170.
    [25] Menof. New method for control of hypertension. S Afr Med J 24: 172-80.
    [26] Bradshaw et al. Thyroid hypofunction in premenstrual syndrome. New Engl Med 1986; 315: 23.

    2001 Serum Thyroglobulin and Urinary Iodine Concentration Are the Most Appropriate Indicators of Iodine Status and Thyroid Function under Conditions of Increasing Iodine Supply in Schoolchildren in Benin1 Tina van den Briel*, Clive E. West2, Joseph G.A.J. Hautvast*, Thomas Vulsma**, Jan J. M. de Vijlder** and Eric A. Ategbo Journal of Nutrition 2001:131, 2701-2706

    * Division of Human Nutrition and Epidemiology, Wageningen University, Wageningen, The Netherlands; Department of Gastroenterology, University Medical Center Nijmegen, The Netherlands; ** Emma Children's Hospital, Academic Medical Center, University of Amsterdam, The Netherlands; and Department of Food and Nutrition, Faculty of Agriculture, National University of Benin, Cotonou, Benin, West Africa


    Abstract:
    Iodine deficiency control programs have greatly reduced iodine deficiency disorders worldwide. For monitoring changes in iodine status,different indicators may be used. The aim of this study wasto evaluate the suitability of indicators of iodine status and thyroid function, thyroglobulin (Tg), thyroid-stimulating hormone(TSH) and free thyroxine (FT4) in serum, thyroid volume and urinary iodine concentration, in iodine-deficient schoolchildren under conditions of increasing iodine supply. The study was established as a double-blind, placebo-controlled oral administration of a single dose of iodized oil to schoolchildren (7–10 y old), living in an iodine-deficient area of Benin, with an observation period of 10 mo. However, 3–4 mo after supplementation, iodized salt became available in the area. The study population therefore comprised an iodized oil–supplemented group and a nonsupplemented group, both of which had variable, uncontrolled intakes of iodized salt during the last 6 mo of the study. Initial mean serum concentrations of TSH and FT4 were within the normal range, whereas serum Tg concentration, urinary iodine concentration and thyroid volume were indicative of moderate-to-severe iodine deficiency. At the end of the study, all indicators had improved significantly, except thyroid volume, which had decreased only in the supplemented group. The supplemented group also still had significantly lower serum Tg and higher urinary iodine concentrations than the nonsupplemented group. Serum Tg and urinary iodine concentrations are the indicators most influenced by a changing iodine supply. Current normal reference ranges of serum concentrations of TSH and FT4 are too wide for detecting iodine deficiency in this age group.

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    1999 IF DEPRESSION TREATMENT ISN'T WORKING, DO A THYROID EVALUATION . (cover story) Psychopharmacology Update, 1999:10, Issue 4, 1


    New research adds more credibility to the practice of augmenting antidepressant therapy with thyroid hormones in depressed patients. Although it is important to emphasize that the study looked only at a population of patients with hypothyroidism, investigators found that partial supplementation with both triiodothyronine T3, Cytomel, Triostat) or thyroxine T4, Levothroid, Synthroid and others) improved mood and neuropsychological function in patients, some of whom were depressed (see table, p. 10).

    The simply did better and felt better when we gave them some T3 in place of some of the T4 -- Arthur J. Prange, Jr., M.D.

    In a double-blind study, investigators compared the effects of thyroxine alone with thyroxine plus triiodothyronine in 33 hypothyroid patients.
    ....
    Patients had lower serum free and total thyroxine concentrations and higher total triiodothyronine concentrations after treatment with thyroxine plus triiodothyronine than after thyroxine treatment alone.
    ....
    Treating normal levels

    Prange warns, however, that thyroid levels may be within normal limits in depressed patients. Even in those patients, giving them 25 mcg of triiodothyronine along with a standard antidepressant drug appears to be effective for depression. If the depressed patient is found to be hypothyroid, then the condition has to be treated in the usual fashion with both hormones.
    ....
    "A euthyroid person can adapt his or her own endogenous secretion, and tolerate bigger doses of thyroid hormone," he adds. "If you're treating hypothyroidism, you start quite cautiously, because you don't want to put somebody into adrenal insufficiency."
    ....
    If depressed patients don't respond to a one-month trial of antidepressant therapy, Prange recommends that they be started on thyroid supplementation. Since about two-thirds of patients will respond in a week or so to T3, that is preferable to switching patients to a second antidepressant, he says.

    Study has 'indirect applications'
    ....
    Johnson says many physicians use only the TSH test to determine if patients are hypothyroid.
    "But in depressed patients with low thyroid hormone levels, the TSH may still be on the low end," he explains. "Total levels of T3 and T4 also are very unreliable clinically. Almost always, they'll be normal, [but] when you check the free levels, they may actually be low. So you have to check the free levels or you'll miss a lot of people with low hormone levels."

    What are 'normal ranges' for thyroid levels?

    .... The normal range for free T4 levels is about 0.7 to 1.9 mcg per ml; for free T3, the normal range is about 2.3 to 4.0 mcg per ml. The normal range for free thyroid levels is divided into four quarters, which are called quartiles. If the free T4 level is either low or in the bottom quartile of the normal range, he prefers to use thyroxine first and then add triiodothyronine later if they don't show improvement. If, however, the free T4 is in the second quartile or above, he will initiate treatment with triiodothyronine only.
    "I generally find that most patients ultimately need both," Johnson says.
    The doses he usually finds helpful are between 5 mg to 25 mcg of triiodothyronine and between 50 mcg and 250 mcg of thyroxine.
    ....
    Reference
    Bunevicius R, Kazanavicius G, Zalinkevicius R, et al.: Effects of thyroxine as compared with thyroxine plus triiodothyronine in patients with hypothyroidism. New England Journal of Medicine 1999; 340:4244-429.
    Cooke RG, Joffe RT, Levitt AJ:T3 augmentation of antidepressant treatment in T4-replaced thyroid patients. Journal of Clinical Psychiatry 1992; 53:16-18.

    2000 The role of brain thyroid hormones in the mechanisms of seasonal changes in mood and behavior Sher Rockville, Maryland, USA Medical Hypotheses Vol. 55, No. 1, July 2000

    Abstract: Many individuals experience seasonal changes in mood andbehavior. Various theories have been suggested to explain the mechanisms of these changes. However, the mechanisms of seasonal mood and behavioral changes remain unclear. The author suggests that brain thyroid hormones may play an important role in seasonal changes in mood and behavior. This suggestion is based on the facts that seasonal changes in light and temperature may affect the metabolism of brain thyroid hormones and that small alterations of the brain thyroid economy, independent of peripheral changes in thyroid status, may produce significant behavioral effects. The author further suggests that there may be a fault in the thyroid metabolism in the brain in seasonal affective disorder patients, and that fault cannot be identified by studying the peripheral thyroid hormone metabolism. Seasonal mood and behavioral changes may also be related to the interaction between thyroid hormones and different neurotransmitter systems in the brain.

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    1998 HUMAN REPRODUCTION AND IODINE DEFICIENCY: IS IT A PROBLEM IN THE UK? Wynn, Margaret; Wynn, Arthur Journal of Nutritional & Environmental Medicine, Mar98, Vol. 8 Issue 1, p53

    Women suffer from thyroid disorders between four and ten times as frequently as men of the same age. This has been shown to be due to iodine deficiency causing damage to the thyroid during pregnancy and is not true of women who have never been pregnant. Iodine deficiency not only damages women but causes impairment of brain development in their babies. These are the main reasons for the recent increase in the World Health Organization (WHO) recommendation for an iodine intake to 200 mcg/day during pregnancy. Many British women have iodine intakes much below this recommendation and indeed below 100 mcg/day. The WHO was, however, informed by the British government that tile population at risk of iodine deficiency in the UK was `zero'. The WHO recommendations for action ore discussed.


    INTRODUCTION

    A paper in this journal by Durrant-Peatfield [1] gave an interesting account of `thyroid dysfunction and management' in clinical practice and suggested that hypothyroidism in particular has a higher prevalence than is generally thought.
    ....
    Pregnancy demands higher thyroid hormone secretion and stresses the thyroid, which may be permanently damaged if the iodine supply is inadequate. This discovery was one reason why the WHO sponsored a conference in Brussels in 1992 under the title `Iodine Deficiency in Europe: a Continuing Concern'. The proceedings have been published [2].
    ....
    Following the 1992 conference the WHO had further meetings at regional and global levels and increased the recommended iodine intake for pregnant women to 200 mcg/day [3]. The present review shows that there are large numbers of women in Britain with iodine intakes below the current WHO recommendations and. indeed, below the recommendations before the present increase. Does this matter? If it does matter, what is to be done?

    THE EFFECTS OF IODINE DEFICIENCY ON THE BRAIN OF THE UNBORN CHILD

    The WHO report emphasizes that iodine deficiency in pregnant women may cause irreversible brain damage in the developing fetus [4]:
    Infants and young children exposed to iodine deficiency may also suffer from brain damage, psychomotor retardation and intellectual impairment. Thus IDD include a broad spectrum of conditions that vary in severity ... iodine deficiency also affects reproductive function, leading to increased rates of abortion. still births, congenital anomalies, low birth weights and infant and young child mortality (p. 2).
    It is the effect of iodine deficiency in a mother on the mental and psychiatric development of her children that has come to be regarded during the last 15 years as of the greatest social importance, exceeding any direct consequences of iodine deficiency in adults. European countries are no longer troubled by extreme iodine deficiency resulting in cretinism, but by lesser degrees of maternal iodine deficiency resulting in losses in the mental ability of their children recorded at school age and expressed as a loss in learning ability. The introduction to a paper by Connolly and Pharoah [5. p. 317] (from the University of Sheffield and University of Liverpool, respectively) discusses hearing loss, motor competence and cognitive function. It states:
    That iodine deficiency diseases present a spectrum of developmental consequences which vary from the gross to those detected only by careful and precise quantitative measures is of considerable significance biologically and socially. The less severe manifestations may in fact be of greater social significance because many more individuals are affected and put at developmental risk.
    ....
    Few clinical studies have been found on the effects of paternal iodine deficiency oil children. A few studies on experimental and farm animals have recently been reviewed 191. In male animals, hypothyroidism is reported to reduce libido and sperm number, motility and density, delay puberty onset and reduce the final testis size. final conception rate and fertility.

    PREGNANCY CAN DAMAGE THE THYROID GLAND

    Women report more thyroid disorders than men. Data from the US National Health Interview Survey 1992 are shown in Table 1 [10]. Statistics understate the prevalence of thyroid disorders. particularly cases of a less serious character which are often not diagnosed or reported. ....

    in a minority of cases of thyroid dysfunction following pregnancy, the thyroid has been shown not to return to normal even several years post-partum. A study from the University of Wales found that 23% of patients with post-partum thyroiditis were hypothyroid 3.5 years post-partum [141. A study from Sweden reported 31% long-term hypothyroidism following post-partum thyroiditis [15].
    Thyroid dysfunction has been reported in women who have had a miscarriage [16]. Iodine deficiency can, in fact, cause miscarriage. In women who are even mildly iodine deficient the thyroid dysfunction may be aggravated by each successive pregnancy.
    Permanent thyroid dysfunction can generally be treated successfully. but the milder disorders in particular are often not treated. Depressed levels of thyroid hormones reduce the body's capacity for protein synthesis and tissue renewal and accelerate ageing [17]. Thyroid dysfunction should be diagnosed and treated.
    ....
    DANGERS OF IODINE FORTIFICATION FOR SUSCEPTIBLE INDIVIDUALS

    The UK chapter in the symposium [2] says nothing at all under the heading `Conclusions' about the effect of iodine intake on pregnancy outcome but says that: "There are no plans for any national regulation of iodine supplementation. Attention has been drawn to the dangers of iodine supplementation in susceptible individuals" (pp. 326-7),
    Iodine supplementation can aggravate thyrotoxicosis in individuals with defective thyroid glands. The tenth edition of the American report Recommended Dietary Allowances suggests that iodine intake only causes thyrotoxicosis in individuals who have been exposed to years of iodine deficiency and who have, in some measure. adapted to a low iodine intake [21]
    .....
    A statement in 1994 by the WHO [22, pp. 6-7] entitled Iodine and Health concluded that:
    Issues relating to the safety of universal salt iodization have been carefully examined by WHO ... The benefits to be derived from universal salt iodization ... and the absence of significant adverse effects among others in the same areas who are not iodine deficient, far outweigh any risk of excess intake for a small minority.

    IODINE DEFICIENCY IN THE UK

    The WHO [4] estimated the number of people probably `at risk' of iodine deficiency in Europe at many millions. The WHO [4] report lists the world's nations and their own estimates of the size of populations `affected' and `at risk' from iodine deficiency diseases. Thus, for example, the report shows the populations affected as being 5% in Belgium, 5% in France, 10% in Germany and 2.5% in The Netherlands. The UK reported to WHO that: "Population affected 0. population at risk 0 ... No national data ... it is generally considered that the iodine status of the general population is adequate" (p. 39). The daily intake of women as recorded by the Dietary and Nutritional Survey of British Adults in 1990 [23] is shown in Table 2 and Fig. 2 [24].
    How do these recorded intakes compare with the recommendations? As can be seen from Table 2 and Fig. 2, a large percentage of women in the UK have iodine intakes well below the allowances recommended by the Department of Health [25]. Further, as evidenced in Table 4 and Fig. 2, the iodine intake of many women in the UK is even further below the recommendations of the WHO [3].
    The main difference between Tables 3 and 4 is in the recommendations for pregnancy and lactation. The UK Dietary Reference Values report says: `Pregnancy--no increment' and `Lactation--no increment'. The WHO recommends an extra 50 mcg/day of iodine during both pregnancy and lactation. The WHO had previously recommended only an extra 25 mcg/day for pregnancy and lactation in line with American recommendations, but the symposium on iodine deficiency in Europe in 1993 recommended an increase in the supplements for pregnancy and lactation to 50 mcg/day [2].

    WHO RECOMMENDATIONS TO ELIMINATE IODINE DEFICIENCY DISEASES

    ....
    The monitoring of the iodine intake of mothers and infants in Europe by periodic analysis of urinary iodine levels, and the measurement of thyroid stimulating hormone (TSH) and other thyroid hormones T4 and T3 `to the extent feasible', are recommended in the symposium. It is also recommended that the "mother's diet should be systematically supplemented with iodine whenever necessary by vitamin/mineral tablets as prescribed by physicians" [2, p. 478].

    THE NEED FOR IODINE BEFORE CONCEPTION

    There are great difficulties in these recommendations because the evidence shows that, to be wholly effective, iodine deficiency has to be corrected before ovulation and conception.
    ....
    Animal experiments have shown that if iodine deficiency begins during the period preceding mating, it causes a much more serious range of congenital malformations than if it begins only a few days after mating [37]. Low T[sub 4] levels, which may be a consequence of iodine deficiency, can cause mutations in males and females that may be inherited in their F[sub 1] offspring and in the following F[sub 2] generation [38, 39].

    HOW CAN IODINE INTAKE BE INCREASED?

    The WHO recommends that the iodization of salt should be introduced by legislation in all countries which have not already done so, as already discussed. There have now been three generations of experience of the iodization of salt, which has been found not to be completely effective. The soundness of the WHO recommendation is not questioned, but implementation leaves some people still iodine deficient.
    The fortification of salt is likely to prove increasingly inadequate as the populations of Britain and other countries respond to the advice that they are eating too much salt for the good of their health.
    ....
    The World Bank, in its 1993 World Development Report, stated that the fortification of foods was one of the most cost-effective public health interventions. A decision to introduce iodine fortification in the UK needs to he preceded by a study of the distribution of iodine deficiency. if it is decided to enrich manufactured foods, a limited number of manufacturers who can provide good population coverage have to be chosen and procedures have to be agreed. Every programme must then be monitored. There are historical examples of the recrudescence of iodine deficiency following the relaxation of monitoring [44]. A programme to increase the intake of iodine in the UK should be under statutory control.

    TABLE 1. Goitre or other disorders of the thyroid gland by age and sex, USA, 1992, nationwide interview sample for National Health Interview Survey
    Cases per 1000 persons

    Under 45 45-64 65-74 75 years
    years years years and over

    Male 3.3 11.4 18.2 9.1
    Female 13.4 53.7 59.0 51.7

    Source: [10, Table 58].

    TABLE 2. Mean daily iodine intake (mcg), Great Britain, 1990
    Men Women

    16-64 16-24 25-34 35-49 50-64 16-64
    years years years years years years

    Median 226 146 158 172 171 163
    Lower 2.5
    percentile 99 61 53 75 67 63

    Source: [23].

    TABLE 3. Dietary reference values (RNIs) for iodine, UK, 1991
    Age RNI (mcg/day)

    0-3 months (formula fed) 50
    4-12 months 60
    1-3 years 70
    4-6 years 100
    7- 10 years 110
    11-14 years 130
    15-18 years 140
    Over 18 years 140

    Pregnancy No increment
    Lactation No increment

    Source: [25].

    TABLE 4. Recommended intakes of iodine (population requirements)[a]
    Intake
    Age range or state (mcg/day)

    0-12 months 50
    1-6 years 90
    7-12 years 120
    12 years to (and through) adulthood 150
    Pregnancy 200
    Lactation 200

    [a] For virtually all practical purposes, these allowances can
    be regarded as serving the same purpose as estimates of
    Population minimum mean intakes sufficient to meet normative
    requirements.

    Source:[3].

    TABLE 5. Women's food sources of iodine, Great Britain, 1994
    Amount Daily
    Food (mcg/day) intake (%)

    Milk and milk products 72 39
    Cereal products 26 14
    Total beverages 18 10
    Fish 15 8
    Confectionery, sugar and preserves 12 6
    Meat and meat products 10 5
    Egg and egg products 9 5
    Potatoes 4 2
    Fruit 4 2
    Vegetables 3 2

    Source: [24].

    TABLE 6. Iodine content of some common foods
    Food mcg 100 g[sup -1] mcg MJ[sup -1]

    Fish (cod) 110 342
    Eggs 53 87
    Whole milk 15 55
    Bananas 8 20
    Meat (beef) 6 8
    Bread (white) 6 6
    Potatoes (old) 3 9
    Cabbage 2 18
    Pulses (peas) 2 6
    Fruit (pears) 1 6

    Source: [40].


    FIG. 1. Thyroid of German and Swedish women, 1986. Source: [20]. Published by permission of the Society of the European Journal of Endocrinology.


    FIG. 2. Daily iodine intake of British women from food, 1994 (n = 1110). Source: [24]. Published by permission of HMSO.

    REFERENCES

    [1] Durrant-Peatfield BJ. Aspects of common missed diagnosis: thyroid dysfunction and management. J Nutr Environ Med 1996; 6: 371-8.
    [2] Delange F, Dunn JT, Glinoer D. Iodine deficiency in Europe: a continuing concern, Vol. 241. New York: Plenum Press, 1993.
    [3] WHO. Trace Elements in Human Nutrition and Health. Geneva: WHO, 1996.
    [4] WHO. Global Prevalence of Iodine Deficiency Disorders. Geneva: WHO, 1993.
    [5] Connolly KJ, Pharoah POD. Iodine deficiency, maternal thyroxine levels in pregnancy and developmental disorders in the children. In: DeLong GR, Robbins J, Condliffe PG, eds. Iodine and the Brain. New York: Plenum Press, 1988; 317-31.
    [6] Churchlll JA, Neff JW, Caldwell DF. Birthweight and intelligence. Obstet Gynecol 1966; 28: 425-9.
    [7] DeLong GR, Robbins J, Condliffe PG, eds. Iodine and the brain. New York: Plenum Press, 1988.
    [8] Hetzel BS. The story of iodine deficiency. Oxford: Oxford University Press, 1989.
    [9] Jannini EA, Ulisse S, D'Armiento M. Thyroid hormone and male gonadal function. Endocrine Rev 1995; 16: 443-59.
    [10] Centers for Disease Control and Prevention. Vital and Health Statistics. Current Estimates from the National Health Interview Survey, 1992. Hyattsville, MD: US Department of Health and Human Services, 1994.
    [11] Struve C, Ohlen S. Einfluss fruherer Schwangerschaften auf Strumen und Knothaufigkeit bei schilddrusengesunden Frauen. Dtsch Med Wschr 1990; 115: 1050-3.
    [12] Glinoer D, Lemone M, Bourdoux P, et al. Partial reversibility during late postpartum of thyroid abnormalities associated with pregnancy. J Clin Endocrinol Metab 1992; 74: 453-7.
    [13] Glinoer D, Nayer P, Delange F, et al. A randomized trial for the treatment of mild iodine deficiency during pregnancy; maternal and neonatal effects. J Clin Endocrinol Metab 1995; 80: 258-69.
    [14] Othman S, Phillips AP, Parkes AB, et al. A long-term follow-up of postpartum thyroiditis. Clin Endocrinol 1990; 32: 559-64.
    [15] Jansson R, Dahlbeg PA, Karlsson AF. Postpartum thyroiditis. in: Lazarus JH, Hall R, eds. Clinical Endocrinology and Metabolism, Hypothyroidism and Goitre, Vol. 2. London: Bailliere Tindall, 1988; 619-35.
    [16] Stagnaro-Green A. Post-miscarriage thyroid dysfunction. Obstet Gynecol 1992; 80: 490-2.
    [17] D'Costa AP, Lenham JE, Ingram RL, et al. Comparison of protein synthesis in brain and peripheral tissue during aging. NY Acad Sci 1993; 692: 253-5.
    [18] Rasmussen SN, Hjorth L., Determination of thyroid volume by ultrasonic scanning. J Clin Ultrasound 1974; 2: 143-5.
    [19] Hegedus L, Perrild H, Poulsen LR, et al. The determination of thyroid volume by ultrasound and its relation to body weight, age and sex in normal subjects. J Clin Endocrinol Metab 1983; 56: 260-3.
    [20] Gutekunst R, Smolared H, Hasenpusch U. Goitre epidemiology: thyroid volume, iodine excretion, thyroglobulin and thyrotropin in Germany and Sweden. Acts Endocrinol (Copenh) 1986; 122: 494-501.
    [21] National Research Council. Recommended Dietary Allowances, 10th edn. Washington, DC: National Academy Press, 1989.
    [22] WHO. Iodine and Health. Geneva: WHO, 1994.
    [23] Gregory J, Foster K, Tyler H, et al. The Dietary and Nutritional Survey of British Adults. Office of Population Censuses and Surveys. London: HMSO, 1990.
    [24] Ministry of Agriculture, Fisheries and Food. The Dietary and Nutritional Survey of British Adults: Further Analysis. London: HMSO, 1994.
    [25] Department of Health. Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. London: HMSO, 1991.
    [26] Burgi H, Supersaxo Z, Selz B. Iodine deficiency diseases in Switzerland one hundred years after Theodor Kocher's survey. Acta Endocrinol (Copenh) 1990: 123: 577-90.
    [27] Pharoah POD, Buttfield IH, Hetzel BS. Neurological damage to the fetus resulting from severe iodine deficiency during pregnancy. Lancet 1971; i: 308-10.
    [28] Pharoah POD, Connolly KJ. Maternal thyroid hormones and fetal brain development. In: DeLong GR, Robbins J, Condliffe PG, eds. Iodine and the Brain. New York: Plenum Press, 1988; 333-54.
    [29] Samuels HH, Forman BM, Horowitz ZO, et al. Regulation of gene expression by thyroid hormone. J Clin Invest 1988; 81: 957-67.
    [30] Chin WW, Yen PM. T[sub 3] or not T[sub 3]--the slings and arrows of outrageous TR function. Endocrinology 1996; 137: 387-9.
    [31] Helmer EB, Raaka BM, Samuels HH. Hormone-dependent and -independent transcriptional activation by thyroid hormone receptors are mediated by different mechanisms. Endocrinology 1996; 137: 390-9.
    [32] Fagin JA, Fernandez-Mejia C, Melmed S. Pituitary insulin-like growth factor-1 gene expression: regulation by triiodothyronine and growth hormone. Endocrinology 1989; 125: 2385-91.
    [33] Wolf MS, Ingbar G, Moses AC. Thyroid hormone and growth hormone interact to regulate insulin-like growth factor-1 messenger ribonucleic acid and circulating levels in the rat. Endocrinology 1989: 125: 2905-14.
    [34] Ceda GP, Fielder PJ, Donovan SM, et al. Regulation of insulin-like growth factor-binding protein expression by thyroid hormone in rat GH3 pituitary tumor cells. Endocrinology 1992: 130: 1483-9.
    [35] Cacicedo L, Frailes MT, Lorenzo MJ, et al. Pituitary and peripheral insulin-like growth factor-1 regulation by thyroid hormone. Ann NY Acad Sci 1993; 692: 287-90.
    [36] Giudice LC. Insulin-like growth factors and ovarian follicular development. Endocrine Rev 1992; 13: 641-69.
    [37] Langman J, van Faasen F. Congenital defects in the rat embryo. Am J Ophthalmol 1955: 40: 65-76.
    [38] Bakke JL, Lawrence NL, Bennett J, et al. Endocrine syndromes produced by neonatal hyperthyroidism, hypothyroidism or altered nutrition and effects seen in untreated progeny. In Fisher DA, Burrow GN, eds. Perinatal Thyroid Physiology and Disease. New York: Raven Press, 1975; 107-12.
    [39] Wauben-Perrls PJJ, von Buul-Offers SC, Meiotic nondisjunction in male Snell dwarf mice. J Hered 1982; 73: 365-9.
    [40] Holland B, Welch AA, Unwin ID, et al. McCance and Widdowson's The Composition of Foods, 5th edn. London: Royal Society of Medicine, Ministry of Agiculture, Fisheries and Food, 199).
    [41] Kaufmann S. Prevention of iodine deficiency disorders in southeast Asia: enrichment of iodine in the food chain. Inst Tierernahrung 1996: 12: 10-12.
    [42] Kaufmann S, Rambeck WA. An additional strategy for the fight against iodine deficiency; iodizing animal feed. In: Braverman LE, ed. Thyroid and Trace Elements. Oxford: Blackwell, 1996; 216-18.
    [43] Symonds ME. Pregnancy, parturition and neonatal development: interactions between nutrition and thyroid hormones. Proc Nutr Soc 1995; 54: 329-43.
    [44] Dunn JT. Extensive personal experience; seven deadly sins in confronting endemic iodine deficiency, and how to avoid them. J Clin Endocrinol Metab 1996: 81: 1332-5.

    research into thyroid updated with:
    (top of page)

    Editorial - Drummond Rennie, MD April 16, 1997 JAMA. 1997;277:1238-1243
    Thyroid Storm
    In this issue of THE JOURNAL, we are publishing a report of work that started 9 years ago, was concluded in December 1990, and the data from which were published in another journal in July 1995. Given that we at JAMA like to keep up-to-date and that we try never to republish what others have already put in print, the reader might well ask what is going on. The story necessary to answer this question provides a cautionary tale that illustrates the sharply differing views of research taken by the university researcher and the company sponsoring that research, if the company's product is at stake. At a time when an increasing proportion of research funding is provided by private companies the story holds lessons for both, as well as for university faculties, administrators, regulatory agencies, and for physicians who prescribe on the basis of evidence.
    In this Editorial, I shall be discussing events that took place at the University of California, San Francisco (UCSF), which is where the West Coast office of JAMA is situated. I should make it plain that until JAMA became involved, I did not know, and had never had contact with, any of the research workers involved.

    Background
    The issue of the potency, reliability, and bioequivalence of levothyroxine preparations has continued to raise controversy. Natural thyroid extracts were marketed before the regulations of 1938 and so were exempted from amendments to the Food, Drug, and Cosmetic Act requiring that drugs be proved safe and effective. Synthroid, the first synthetic version, had come to dominate a $600 million a year market that was essentially unregulated because the Food and Drug Administration (FDA) had no approved standards for bioavailability and bioequivalence and no mechanism to evaluate them, and there were no adequate well-controlled trials. Such dominance was unusual, given that other competing formulations of levothyroxine had been available for years, and it was greatly assisted by the manufacturer's claims that other preparations were not bioequivalent.
    In 1987, to establish that Synthroid was truly more effective than competing preparations, Flint Laboratories, then the manufacturers of Synthroid, approached Betty J. Dong, PharmD, at UCSF. This seemed a good choice because in 1986, Dong et al had published a letter showing that the levothyroxine content of different thyroid products, 2 brand-name products and 7 generic, differed widely. They noted that the 2 brand-name preparations, 1 of them Synthroid, were the preparations of choice. Flint and Dong signed a lengthy protocol/contract to finance comparative studies of the bioequivalence of Synthroid and 3 other preparations, and both sides expected the study to show that Synthroid was superior (letter from B. J. Dong to N. M. Kurtz, March 31, 1994). The contract detailed the experimental design and analysis of the data. Representatives of Flint, and after their takeover, Boots Pharmaceuticals Inc, made regular site visits, about 3 a year, to satisfy themselves that the work was being done properly. During these visits small problems were ironed out, but there was no hint of any bigger cloud.
    In January 1989, at a time when there was a move to add a competitor's preparation to the Massachusetts formulary,[4] Boots, in the first of their site visits, began asking for the preliminary results of a parallel in vitro study in which tablets were compared, and because this would have meant breaking the masking code and therefore invalidating that particular study, Dong et al refused to comply. By the end of 1990, the major in vivo study was finished, and Dong sent all the results to Boots: it was clear that all 4 preparations were bioequivalent.
    Over the next 4 years, Boots waged an energetic campaign to discredit the study and prevent publication of the drafts Dong and her colleagues sent to them for comment, claiming that the study was seriously flawed. Boots cited scores of purported deficiencies, including failure to carry out procedures not called for in the protocol.
    ....
    Boots had alleged numerous breaches of research ethics, but when asked by UCSF to make specific allegations that UCSF could formally investigate, Boots did not respond. Noting that all records and data had been open to Boots, who had monitored the study closely, UCSF told Boots, in August 1994, that there was no reason to suppress the manuscript and to do so would be an unprecedented intrusion upon academic freedom (letter from P. Lurie and S. M. Wolfe to D. A. Kessler, May 29, 1996). Later, they agreed to meet again with Boots, but suggested that this time it should be in the presence of officials from the FDA. That meeting never took place. Dong et al made numerous changes in their manuscript to accommodate Boots, but finally decided they would publish.
    ....
    What Are the Lessons?
    For Researchers and Faculty.—
    Even if researchers have been approached by sponsors, investigators should not assume that the sponsors will encourage publication of unfavorable results and should never allow sponsors veto power. Dr Dong was naive, but faculty members are the last line of defense against industry interference, and she and her colleagues deserve credit for standing up for their academic rights.
    ....
    For the FDA.—Thyroid preparations were grandfathered in by the 1938 Food, Drug, and Cosmetic Act, which required demonstration of safety, and the 1962 amendment, which required that drugs be shown to be effective. As is the case with other preparations of levothyroxine, Synthroid, introduced in 1958, could reasonably be regarded as a reformulation. The FDA has the authority to designate important pre-1938 drugs that have been reformulated as "new" drugs and require a New Drug Application (NDA).
    ....
    A simpler and possibly more fruitful approach to setting standards for both bioequivalance and clinical interchangeability might be for scientific organizations with the best expertise in this area, such as the American Association of Pharmaceutical Scientists, the American Society for Clinical Pharmacology and Therapeutics, and the American Thyroid Association, to establish guidelines by consensus, which they could then publish for the benefit of all.
    ....
    Is This Common?
    The Synthroid case, where publication was delayed about 7 years, seems an extreme case. However, in this issue of THE JOURNAL, we publish a paper from Blumenthal et al on withholding of research results by researchers. These authors found that almost 20% of 2100 life science faculty reported delay of over 6 months in the publication of their research results.
    ....
    if "undesired results" are withheld by only about 5% of all researchers, the fears induced by the increased part industry is playing in the funding of research are not dispelled. And before we decide the danger is past, workers at Carnegie-Mellon University reported that in their sample of university-industry research centers, 35% of the signed agreements allowed the sponsor to delete information from publication, 53% allowed publication to be delayed, and 30% allowed both.
    ....
    Rosenberg,sounding the alarm, makes the point that secrecy in research is increasing and gives 4 examples from his personal experience. He writes: "The goals of medical research are clear: to prevent human suffering and premature death from disease.... Deliberately withholding useful information ... is a violation of this principle." As I have pointed out before, 25 a major problem in medicine is failure to publish the results of studies that show no advantage to the intervention under study, so that treatments tend to be based on biases in favor of the new. I take Chalmers' position that it is unethical not to publish such negative results. The Olivieri case, hinging as it does on the interpretation of data about the safety of a therapy, shows that this is not just a theoretical position.
    Rosenberg[24] concludes, as do I, that scientists should never sign any agreements that give their sponsors veto power over publication.
    Marshall has recently described the battle in genome research between those who wish to lock up results by delaying publication and those, including sponsors both governmental and commercial, who see a wider societal good in putting gene sequences promptly into the public domain. Marshall notes that, for example, withholding DNA sequence data on pathogens could cost human lives, but is "commonplace." It is too early to see who will win, but unless the scientific community gives its strong support and approval to sponsors who forbid secrecy, we will all suffer the consequences.

    Conclusion
    We are proud to publish the article by Dong and her colleagues. We believe it is good work, not merely because it passed peer review by more than the usual number of experts, but because it has also passed careful and prolonged scrutiny by the university in response to widely disseminated allegations of scientific defects and ethical violations. We are also confident in the work because of the university's finding that none of the allegations had the slightest merit and because they came from those who had most to gain if the work was discredited. Now that the thyroid storm has passed, clinicians and third-party payers finally have the information they need to best serve their patients.

    ....

    References
    1. Dong BJ, Hauck WW, Gambertoglio JG, Gee L, White JR, Bubp JL, Greenspan FS. Bioequivalance of generic and brand-name levothyroxine products in the treatment of hypothyroidism. JAMA. 1997;277:1205-1213.
    2. Blumenthal D, Causino N, Campbell E, Louis KS. Relationships between academic institutions and industry in the life sciences: an industry survey. N Engl J Med. 1996;334:368-373.
    3. Cooper DS. Thyroid hormone treatment: new insights into an old therapy. JAMA. 1989;261:2694-2695.
    4. King R. Bitter pill: how a drug company paid for university study, then undermined it. Wall Street Journal. April 25, 1996:1.
    5. Dong BJ, Young VR, Rapaport B. The nonequivalence of thyroid products. Drug Intell Clin Pharm. 1986;20:77-78.
    6. Benet LZ. Morality play. Science. 1996;273:1782.
    7. University of California Contract and Grant Manual. 1-340 Guidelines on University-Industry Relations, May 17, 1989.
    8. University of California Contract and Grant Manual. 11-110.
    9. Kenyon G, Drake S. Statement regarding contract for clinical trials. University of California, San Francisco, February 27, 1996. Press release.
    10. Mayor GH, Orlando T, Kurtz NM. Limitations of levothyroxine bioequivalence evaluation: analysis of an attempted study. Am J Ther. 1995;2:417-432.
    11. Berg JA, Mayor GH. A study in normal human volunteers to compare the rate and extent of levothyroxine absorption from Synthroid and Levoxine. J Clin Pharmacol. 1992;32:1135-1140.
    12. Eckert C. Bioequivalence of levothyroxine preparations: industry sponsorship and academic freedom. JAMA. 1997;277:1200.
    13. Spigelman MK. Bioequivalence of levothyroxine preparations for treatment of hypothyroidism. JAMA. 1997;277:1199.
    14. Dong BJ, Hauck WW, Gambertoglio JG, Gee L, White JR, Bubp JL, Greenspan FS. Bioequivalence of levothyroxine preparations: industry sponsorship and academic freedom. JAMA. 1997;277:1200-1201.
    15. Dong BJ, Hauk WW, Gambertoglio JG, Gee L, White JR, Bubp JL, Greenspan FS. Bioequivalence of levothyroxine preparations for treatment of hypothyroidism. JAMA. 1997;277:1199-1200.
    16. Zinberg DS. A cautionary tale. Science. 1996;273:411.
    17. Eckert C. Morality play. Science. 1996;273:1784.
    18. Blumenthal D, Campbell EG, Anderson MS, Causino N, Louis KS. Withholding research results by academic life scientists: evidence from a national survey of faculty. JAMA. 1997;277:1224-1228.
    19. Cohen W, Florida R, Goe WR. University-Industry Research Centers in the United States. Pittsburgh, Pa: Carnegie-Mellon University Press; 1994.
    20 Olivieri NF, Brittenham GM, Matsui D, et al. Iron-chelation therapy with oral deferipronein in patients with thalassemia major. N Engl J Med. 1995;332:918-922.
    21. Nathan DG. An orally active iron chelator. N Engl J Med. 1995;332:953-954.
    22. Olivieri NF. Randomized trial of deferiprone (L1) and deferoxamine (DFO) in thalassemia major. Blood. 1996;88(suppl 1):651a.
    23. Jeffrey S. Research conflict. Med Post. 1997;33:1.
    24. Rosenberg SA. Secrecy in medical research. N Engl J Med. 1996;334:392-394.
    25. Rennie D, Flanagin A. Publication bias: the triumph of hope over experience. JAMA. 1992;267:411-412.
    26. Chalmers I. Underreporting research is scientific misconduct. JAMA. 1990;263:1405-1408.
    27. Marshall E. Is data-hoarding slowing the assault on pathogens? Science. 1997;275:777-780.

    2 1997 Bioequivalence of levothyroxine preparations Dong BJ, Hauck WW, Gambertoglio JG, Gee L, White JR, Bubp JL, Greenspan FS. Bioequivalence of generic and brand-name levothyroxine products in the treatment of hypothyroidism. JAMA 1997; 277: 1205-1213.
    Reviewed by
    Kenneth G. Schellhase, MA, MD and Allan Ellsworth, PharmD
    Clinical question
    Can different commercial preparations of levothyroxine be used interchangeably in the treatment of hypothyroidism?
    Background
    An estimated 8 million prescriptions for thyroid replacement are written yearly in the U.S. Most of these prescriptions are written for the Synthroid brand of levothyroxine due to perceived lack of quality or therapeutic equivalency of generic versions. In vitro bioequivalency data are available for several brand-name products, but the bioequivalence of generics has not been tested. In this in vivo study, therapeutic equivalency of two brand-name and two generic levothyroxine preparations was compared.
    Population studied
    Twenty-four women taking either 0.1 mg or 0.15 mg of levothyroxine were studied. Inclusion criteria included at least two sets of normal thyroid function tests performed at least six weeks apart. Exclusion criteria included conditions known to interfere with the metabolism, absorption, or measurement of levothyroxine. Two subjects were excluded from the final analysis due to protocol violations.
    Study design and validity
    The study was a randomized, single-blind (primary investigators only) four-way crossover trial comparing bioavailability of two brand-name versions of levothyroxine (Synthroid(r) and Levoxyl(r)) and two generic products (distributed by Geneva Generics and Rugby Laboratories, though manufactured by the same company). Subjects were assigned to one of four distinct four-drug sequences. Each product was taken for a minimum of six weeks. Thyroid indices were determined at three-week intervals, and conventional bioavailability parameters were obtained at the end of each six week block. Medication and protocol compliance was monitored. The crossover study design, in which each patient served as her own control, is very strong and allows conclusions to be drawn from a small number of patients. However, generalization to other brands of levothyroxine that were not a part of this study may not be valid.
    Outcomes measured
    The main outcomes were conventional bioavailability parameters at steady state of total thyroxine, triiodothyronine, and resin thyroxine uptake, as well as thyrotropin levels. Dosage forms of the same drug are considered bioequivalent when they yield neither clinically nor statistically significant differences in these parameters.
    Results
    There were no statistically significant differences between these four levothyroxine products at either dose for total thyroxine, total triiodothyronine, and free thyroid index. Moreover, all four preparations produced substantially less variation in bioequivalence (-5% to +7%) than the standard of -20% to +25% allowed by the Food and Drug Administration. The statistical power of this study was high enough to find a difference among the products if one truly existed.
    Recommendations for clinical practice
    This well-executed study provides strong evidence for the bioequivalence of common name-brand and generic forms of levothyroxine. Although the study reports disease-oriented rather than patient oriented evidence, it is clearly of clinical interest. Practitioners can now prescribe these generic formulations with confidence that they will provide reliable, effective, and equivalent therapy. The financial impact of this study could be substantial. The authors estimate that if generics or Levoxyl(r) (which is close to generic cost) were used for roughly half of levothyroxine prescriptions each year, more than $350 million could be saved. The financial ramifications of this study were not lost on the study sponsor, the manufacturer of Synthroid(r). An editorial with this study details how publication of this work was delayed for six years by a dizzying variety of specious complaints from the manufacturer, ranging from flaws in study design to vague suggestions of ethical misdoings.(1) The results of this study were improperly published by the company in another journal, complete with a conclusion more favorable to Synthroid but failing to acknowledge the original investigators.(2) Sadly, this cautionary tale does not represent mere anecdote. As Blumenthal et al. showed, academic-private sector partnerships are significantly associated with delays in publication.(3) While the withholding of research results is far from commonplace, the increasing frequency of academic-private partnerships may merit greater scrutiny to ensure academic freedom is not impaired.
    References
    1. Drummond R. Thyroid Storm (editorial). JAMA 1997; 277: 1238-1243.
    2. Mayor GH, Orlando T, Kurtz NM. Limitations of levothyroxine bioequivalence evaluation: analysis of an attempted study. Am J Ther 1995; 2: 417-432.
    3. Blumenthal D, Campbell EG, Anderson MS, Causino N, Louis KS. Withholding research results in academic life science. JAMA 1997; 277: 1224-1228.

    1997 SCIENTIFIC AND ETHICAL FOUNDATIONS OF NUTRITIONAL AND ENVIRONMENTAL MEDICINE Journal of Nutritional & Environmental Medicine, Dec97, Vol. 7 Issue 4, p219

    Part III: Pharmacodoxy--The Teaching of Pharmacotherapeutics as a First Line of Treatment in Clinical Medical Practice; A: Consideration of Hippocratic and Darwinian-Evolutionary Principles


    This paper addresses the teaching of pharmacotherapeutics as a first line of treatment and coins the term `pharmacodoxy' to describe that approach. It challenges the wisdom and validity of the approach, on the basis of Hippocratic and Darwinian-evolutionary principles, and suggests that doctors make themselves familiar with the large corpus of published knowledge which indicates that nutrient deficiencies, toxic challenge and food intolerances, as well as other factors, can give rise to a whole range of clinical conditions that fall into all medical specialties. It states that adherence to pharmacodox principles in the face of less hazardous therapeutic interventions may be a violation of the prime principle of ethical clinical medical practice, which is `First do no harm'.

    ....
    THE GROWTH OF THE PHARMACEUTICAL INDUSTRY AS A REFLECTION OF DRUG-PRESCRIBING PATTERNS

    A major cornerstone of modern medical therapeutics is the prescribing of pharmaceutical preparations--more often than not, entirely novel man-made chemicals that were not necessarily present during the evolution of our genes.
    In the late 1940s and early 1950s, medicine saw a dramatic increase in the prescribing of pharmaceutical preparations, following the arrival over a few short years of three major medical therapeutic advances: penicillin in the treatment of a number of previously untreatable infectious diseases (a graphic account of the remarkable way in which the advent of penicillin transformed the clinical management of pneumococcal pneumonia is to be found in the introduction to a fascinating book on the effects of electromagnetics on health by Becker [3]); cortisone for inflammatory conditions; and chlorpromazine for psychotic conditions, including schizophrenia. These three major new therapeutic agents dramatically transformed the face of medicine, and medicine truly entered what could be termed its `pharmacodox era'.
    That we are in the `pharmacodox era' is clearly evidenced by the annual expenditure on pharmaceutical drugs by the National Health Service (NHS) in the UK, which in 1995 was Ł 4982 million (approx. US$787] million) [4]; this represents an almost doubling of the percentage of the gross domestic product (GDP) expended on pharmaceuticals by the NHS in the UK over the last 25 years [4]. Between 1970 and 1995, the total number of prescriptions written by doctors in the NHS increased by about 80%,....
    TWO HIPPOCRATIC PRINCIPLES: `FIRST DO NO HARM' AND `ASSIST NATURE'

    The straight teaching addressed in this paper is based on two fundamental Hippocratic principles, which have nothing whatsoever to do with technical advances, and are therefore not touched by the idea of `technical out-of-dateness': `First do no harm' and `Assist nature'. To evaluate the pharmacodox paradigm, i.e. the philosophical and technical basis upon which pharmacodoxy is founded, is a particularly illuminating process when done with particular reference to these two principles.
    While the vast majority of doctors enter the medical profession out of a genuine and admirable vocational desire to help their fellow men and alleviate suffering, it is the author's contention that these two basic Hippocratic principles appear not to have been embraced by pharmacodox doctors who consider prescribing novel pharmaceutical substances as the first line of treatment for the vast majority of the catalogue of human ailments; that is to say `A pill for every ill'. (`Novel' in this context means `not present in the environment during the evolution of the human genome'.)
    ....
    It is impossible to quantify the number of adverse reactions to medically prescribed drugs that occur worldwide, but none of us in medical practice would have been able to go through medical school without witnessing several instances. In a career spanning 40 or 50 years, a busy, conscientious physician will have seen dozens, if not hundreds, of patients suffering from adverse reactions to one or more drugs; naturally, the more alert a physician is, and the more willing to play devil's advocate to one's own treatment, the more adverse reactions will be observed.
    ....
    The physician's awareness of the potential benefit of a new drug is almost entirely based upon the promotional activities of the drug company that produces it. If you, as a patient, are prescribed that drug, and happen to be the one in however many patients who dies as a direct consequence, it would probably be regarded by your doctor, and his or her peers, as `unfortunate', `bad luck' or `idiosyncratic'. In any event, it is difficult to reconcile the outcome for that particular patient with the Hippocratic principle of `First do no harm', whatever the arguments presented to justify that prescribing action.
    .... .....
    The thalidomide tragedy resulted in drug regulation as we now know it. Lip service is paid to the idea that there is no such thing as a safe drug, but the pharmaceutical industry has largely avoided obtaining systematic information on drug use in pregnancy [16], and pregnant women are still prescribed drugs on a regular basis.
    Yes, lessons have been learnt about avoiding such overt, readily discernible consequences of the practical application of the philosophy of pharmacodoxy, as in the phocomelia from thalidomide, but evidence of the subtler, but nevertheless still devastating, disruptions of normal gene expression, cell division and metabolic pathways may only emerge after many patients have been prescribed the drug and when epidemiological studies can be done as, for example, in the New Zealand asthma mortality epidemic cited earlier, by which time many patients may have been harmed or killed.
    In September 1997, the Food and Drug Administration (FDA) in the US issued an `approvable letter' to one of a number of drug companies wishing to market thalidomide in the treatment of erythema nodosum of leprosy, AIDS-related cachexia, aphthous ulcers and graft-versus-host disease [23].
    The idea underpinning pharmacodoxy, as it is still currently practiced, that novel man-made chemicals can be given to patients as a first line of treatment, is still basically the same philosophy that gave rise to the thalidomide tragedy in the 1960s, and it is still a fundamentally dangerous approach, and difficult to reconcile with the idea of `First do no harm'. NSAIDs, asthma inhalers and thalidomide are just three categories of pharmacodox treatments which have evidently caused harm and have resulted in many deaths; many more examples could be cited. A full evaluation of the total number of deaths directly attributable to all prescribed drugs would undoubtedly reveal disturbing statistics.
    Before proceeding to a discussion of the relationship between pharmacodoxy and the second Hippocratic principle of `Assist nature', it would be helpful to bring to mind the mechanisms of disease processes from the viewpoint of evolutionary theory, and the intrinsic concepts employed in the nutritional and environmental medical approach to the clinical management of ill-health and disease processes.

    THE DARWINIAN-EVOLUTIONARY CONTEXT OF PHARMACODOXY

    ....
    Examples of Drug Prescribing as Violating the Principle of `Assist Nature'
    Having briefly summarized the Darwinian evolutionary approach, we are now in a position to discuss the relationship that pharmacodoxy has with the second of these Hippocratic aphorisms: `Assist nature'. Intrinsic in this concept is the attribution of the wisdom of nature. Pervasive in modern medical thinking is what can be regarded as a form of arrogance: introducing a novel (man-made) pharmaceutical molecule, which does not exist in nature, can do what nature cannot do. This is a cornerstone of the tacit philosophy underpinning the pharmacodox approach.
    Unlike the nutritional and environmental medical approach to the assessment of the patient and the underlying molecular mechanisms of disease, the pharmacodox approach gives little or no consideration to whether or not there are adequate quantities of naturally occurring molecules for optimum metabolic and system function (essential nutrients), or to the presence of excess amounts of toxic molecules.
    ....
    The degree to which profit motivation drives the conceptual approach to the treatment of disease, and the widespread introduction of toxic novel chemicals into the environment and the human food chain, will be the subject of a future editorial.
    In most developed countries, doctors prescribe new drugs that have been approved by regulatory authorities, and it is usually only after a period of time, when it becomes evident that there are serious side-effects, that the drug is removed from the market, and after many thousands of patients have been prescribed it. The inevitable consequence of this approach to prescribing novel man-made pharmaceuticals is exemplified by the case of practolol (one of the first beta-blockers). A long time after it was introduced, and after many tens of thousands of patients had been taking it, it was realized that the drug caused severe eye problems and, in some cases, blindness, and it was finally withdrawn from the market.
    ....
    D. R. Laurence, past Professor of Pharmacology at University College Hospital Medical School, London, is the author of a standard medical student textbook of pharmacology [32], in which he describes the oscillations in the development of a drug, where initial enthusiasm for a new drug results in widespread prescribing, then recognition of adverse effects leads to reduced prescribing and the level evens out to a point where the drug is regarded as `the treatment of choice in selected cases'.
    It is hoped that, in the context of the history of medicine, there will be an oscillation in pharmacodoxy, in which prescribing patterns shift from the current high rate of prescribing to a more judicious level, in a pattern similar to that described for a new drug by Laurence.
    ....
    THE CHEMICAL INDUSTRY AND THE PHARMACEUTICAL INDUSTRY

    Not every doctor knows that the pharmaceutical companies that produce potentially toxic and lethal pharmaceuticals are the same companies that produce highly toxic agrochemicals designed to kill life forms (pesticides, herbicides, fungicides, etc.), and that they produce the novel chemical food additives (e.g. tartrazine) known to produce adverse health effects in susceptible individuals.
    ....
    ENTRENCHMENT IN PHARMACODOXY AND `BLINDING' OF RELEVANT DIAGNOSTIC PROCESSES, MISSED DIAGNOSES AND THE LIMITING OF CONSIDERATION OF RELEVANT THERAPEUTIC OPTIONS
    The pharmacodox approach, with its `pill for every ill' attitude, inevitably results in a blinding of the available therapeutic options, with a failure to consider both the predisposing and the precipitating factors (one meaning of the double-blind approach) of ill-health and disease.
    Examples of Pharmacodoxy as the Prevailing Influence Over Therapeutic Options and Decision-making
    The following examples illustrate how the pharmacodox approach can result in misdiagnosis, inappropriate drug treatment and risk to the patient-in other words, bad medicine.
    Case history 1: Saturnine (relating to the toxic metal lead) gouty, hypertensive nephropathy owing to a lead-containing hair preparation. RW is a 60-year-old male with unwanted effects of his treatment for high blood pressure (beta-blocker and diuretic), which his GP had told him he would have to take for the rest of his life. He was also on a NSAID for his polyarthalgia. He complained of malaise, depression and impotence, all of which he said had appeared since he started his medications.
    ....
    This case raises the question of how many other people with hypertension and gout as a result of lead poisoning from lead acetate in hair blackeners such as Grecian 2000 and Morgan's Pomade, and other sources of environmental lead, are being treated, possibly inappropriately as here, with antihypertensives, diuretics and anti-inflammatory drugs.
    Case history 2: Diabetes mellitus and chromium and other micronutrient deficiencies. The prevalence of diabetes mellitus in industrialized society has reached almost epidemic proportions, and pharmacodox treatment focuses on controlling blood glucose levels and treating cardiovascular problems with medication with regular follow-up to detect the onset of complications. Often, nothing is done to remedy the nutrient deficiencies associated with glucose dysmetabolism [39]. The following case illustrates [40]. A 30-year-old nurse had been insulin dependent since she was 12 years old, and suffered from insulin oedema, a condition whereby diabetics develop massive fluid retention at the insulin dosage required to maintain good blood glucose control. The patient also described severe fatigue and malaise when she experienced this phenomenon. A nutritional biochemistry work-up revealed wildly abnormal low levels of red cell membrane omega-3 and omega-6 EFAs, extremely low levels of several trace elements including zinc, manganese, selenium and chromium, as well as very low levels of magnesium and potassium, and a glycosylated haemoglobin (HbA1) of 22.8% (normal: < 8.5%), indicating that she had very poor blood glucose control.
    ....
    There is a substantial body of evidence, excellently reviewed by Werbach [39], indicating that poor status of many trace elements, vitamins, EFAs and amino acids is found in diabetes mellitus, which contribute not only to the impairment of glucose control but also to the many complications of diabetes mellitus. Failure to take heed of this body of literature in relation to the treatment of diabetes mellitus and the prevention of complications, and therefore failure to ensure that such nutrient inadequacies are corrected, compromises the well-being of millions of diabetic sufferers alive today.
    Case history 3: Thiamin-responsive congestive cardiac failure in the elderly. When elderly patients are admitted to hospital in congestive cardiac failure (CCF), standard pharmaceutical treatment is given, to which the patients sometimes fail to respond fully. This is put down to `pump failure', for whatever reason, and little more is done, apart from possibly adjusting the drug regime. Yet it has long been known that these patients may be on a poor diet, especially if they live on their own, and can be markedly vitamin B[sub 1] deficient, and that their CCF is, in fact, wet beri-beri and responds dramatically to intravenous vitamin B[sub 1]. However, many digitalis and diuretic non-responsive CCF patients are often not tested for or given a therapeutic trial of intravenous vitamin B[sub 1] for their condition, as well as other nutrients essential for optimum cardiac function such as chromium, manganese, carnitine, etc.
    These are just three examples of how pharmacodox training, with the thought modality `what is the diagnosis, and what is the drug treatment of choice?', results in a failure to address what is really going on with the patient. There are many more examples, beyond the scope of this paper, of the `conventional' pharmacodox approach failing to address the patient's real problems, such as nutrient deficiencies and food intolerances, and other factors, which can give rise to a range of clinical conditions such as ulcerative colitis, Crohn's disease, asthma, eczema, migraine, gynaecological, endocrine and psychiatric conditions, and other system disorders [39, 41-45]. Such failure results, in many instances, in denying the patient the best and safest available treatment.

    THE DESIRE OF THE PHYSICIAN TO `HELP' THE PATIENT `AT ALL COSTS'

    There is a natural and laudable willingness on the part of doctors to try to alleviate the suffering of the patient as soon and as effectively as possible.
    ....
    The combination of the doctor's desire to help the patient, albeit well intentioned, and the profit-motivated marketing techniques of the pharmaceutical industry is a powerful one, and one which does not necessarily serve the best interests of the patient, but can lead to the pharmaceutical-chemical industry becoming one of the wealthiest and most powerful industries in developed countries.

    TME DIAGNOSIS-DRUG PARADIGM AS A BARRIER TO THINKING

    The diagnosis-drug paradigm is the thought matrix adopted by many of the practitioners of modern medicine, and simply means that once the diagnosis is made, the most common mental mechanism that comes into play is `What drug do I prescribe for this?' This limited thought process leads to a pronounced barrier to thinking clearly about the individual patient, and thereby acts as an inhibitor of understanding, and therefore, inevitably, of the quality of care that a physician can give the patient.
    ....
    Definitions and descriptions of drug action, in drug company promotional literature, are usually limited to the mechanism of action of the pharmaceutical being promoted, without reference to the broader molecular, cellular and systemic mechanisms involved. An excellent example of this is the extremely potent calcium channel-blocking effects of manganese and magnesium, two trace elements that are often present in inadequate amounts for optimum metabolic function. Instead of being advised to make good the deficiencies of these potent calcium channel-blocking essential nutrients, we receive high-intensity expensive advertising geared towards persuading us of the need to prescribe one or more expensive, potentially toxic, pharmaceuticals. This leads to a dysperception of the available therapeutic options: the diagnosis is `A', the drug treatment of choice for this condition is drug `B'. This sort of thought mechanism, entrenched in modern pharmacodoxy, while it may lend itself to the short consultation time (an important consideration when one is trying to understand how prevalent the pharmacodox approach has become), does not require one to consider `what set of genetic, nutritional and environmental challenges have caused this individual's adaptive mechanisms to become exhausted, thereby causing illness, and why'.
    ....

    MEDICAL STUDENT INDOCTRINATION INTO PHARMACODOXY

    Entering medical school with the two principles `First do no harm' and `Assist nature' clearly in mind results in somewhat of a culture shock for most sensitive, aspiring physicians. It is not long before medical students see patients on the wards who have been on long-term drugs with on-going progression of their disease; or some poor patient who is desperately sick as a direct result of polypharmacy-the prescribing of two or more drugs simultaneously-or patients with no hair as a direct result of chemotherapy for malignant disease, for example.
    It is also not long before the young medical student, full of ideals and hopes to alleviate suffering, cure disease and save mankind, is exposed to the full indoctrination process of pharmacology and pharmacotherapeutics. Without passing the various exams in these subjects, the student will never become a doctor. The amount that the student has to learn on the subject of drugs and drug prescribing means that other therapeutic options, other than surgery or radiotherapy or chemotherapy, hardly receive a mention in the medical school curriculum, or in postgraduate medical education. Furthermore, there is another mechanism operant, which causes aspiring physicians to fall into the prevailing paradigm: as stated by Jaffe "... thinking that diverges from the consensus is actively dissuaded by their seniors and mentors as dangerous to their career advancement and hazardous to their receiving grant support" [48].
    A further point of concern when pursuing the potentially hazardous course of drug prescribing is that the potential confusion owing to the sheer number of drugs available for the clinician to prescribe can also put the patient at risk from incorrect prescribing. A recent report of a prescribing surveillance programme in one particular teaching hospital over a 9-year period reported an increase in prescribing errors from 522 in 1987 to 2115 in 1995 [49].

    CONCLUSIONS

    When the only tool you have is a hammer, everything starts looking like a nail. In medicine, when the only (or main) tool you have is pharmacotherapeutics, every condition tends to look like something to be treated with a drug. It could be said that, in the light of the two Hippocratic principles cited in this paper, the truly `orthodox' physicians are the ones who ascribe to the principles of `First do no harm' and `Assist nature', and that physicians entrenched in pharmacodoxy, to the exclusion of almost every other therapeutic option, are a `bunch of drug-prescribing renegades'. This viewpoint may make many pharmacodox physicians feel uncomfortable, especially the majority who entered the noble profession of medicine to alleviate suffering, and who have fallen victim to the pharmacodox paradigm as a result of the powerful drug-oriented consensus.
    A common response, either to criticism of one's lifelong vocational activity or to the arrival of a shift in thinking away from the entrenched paradigm [5, 20, 48], is to attack since, in the words of Thomas Kuhn, such a shift is "generally preceded by a period of pronounced professional insecurity" [50]. Compounding this professional insecurity is the threat to pharmaceutical company vested interests in maintaining the prescribing rate of doctors. It is, perhaps, for this reason that non-pharmacodox approaches are generally looked down upon, denigrated, or even attacked overtly or covertly by pharmacodox doctors and by those whose income comes directly from the pharmaceutical industry.

    A CRI DE COEUR TO PHARMACODOX PHYSICIANS

    ....
    In medicine it cannot be denied that the well-being of the individual patient is the central issue, and that the prescribing of potentially hazardous, if not potentially lethal, drugs is inappropriate and unethical if more effective and less dangerous therapeutic options are available. No longer can pharmacodoxy, demanding evidence-based treatment strategies, disregard the nutritional and environmental medical approach, simply on the grounds that they are only familiar with the pharmacodox-based published literature, and that no other published literature has any relevance. The time has come for pharmacodoxy to realize that ignorance of existing knowledge is no excuse for prescribing toxic, novel pharmaceuticals, which is a violation of the prime principle of ethical clinical medical practice, which is, for the last time in this paper, cited: `First do no harm'.

    REFERENCES

    [1] Davies S. Scientific and ethical foundations of nutritional medicine. Part I: Evolution, adaptation and health (editorial). J Nutr Med 1992; 3: 227-47.
    [2] Davies S. Scientific and ethical foundations of nutritional and environmental medicine. Part 11: Further glimpses of `the higher medicine' (editorial). J Nutr Environ Med 1995; 5: 5-11.
    [3] Becker RO. Cross Currents--The Promise of Electromedicine and the Perils of Electropollution. Los Angeles, CA: Jeremy P Tarcher, 1990.
    [4] Compendium of Health Statistics, 10th edn, Office of Health Economics; PHARMA--Facts & Figures. London: The Association of British Pharmaceutical Industries, 1997.
    [5] Davies S. Downing D. Truth, ethics and consensus--their relation to medical progress and the quality of patient care (editorial) J Nutr Med 1992; 3: 91-8.
    [6] Declaration of Geneva. Geneva: The World Medical Association, 1948. (Subsequently amended, Ferney-Voltaire, France; reprinted J Nutr Med 1992; 3: 153).
    [7] Allison MC, Howatson AG, Torrance MB, et al. Gastrointestinal damage associated with the use of non-steroidal anti-inflammatory drugs. N Engl J Med 1992; 327: 749-54.
    [8] Bjarnason I, Prouse P, Smith T, et al. Blood and protein doss via small-intestinal inflammation induced by non-steroidal anti-inflammatory drugs. Lancet 1987; ii: 711-14.
    [91] Lee SH, Fawcett V, Preece JM. Aplastic anaemia associated with Piroxicam. Lancet 1982; i: 1186.
    [10] Newman NM, Ling RSM. Acetabular bone destruction related to non-steroidal anti-inflammatory drugs. Lancet 1985; ii: 11.
    [11] Rodruigez LAG, Guttham SP, Walker AM et al. The role of non-steroidal anti-inflammatory drugs in acute liver injury. Br Med J 1992; 305; 865--8.
    [12] Wilcox CM, Alexander LN, Cotsonis GA, et al. Non-steroidal anti-inflammatory drugs associated with both upper and dower gastrointestinal bleeding. Dig Dis Sci 1997; 42: 990-7.
    [13] Tannenbaum H, Davis P, Russell AS, et al. An evidence-based approach to prescribing NSAIDs in musculoskeletal disease: a Canadian consensus. Canadian NSAID Consensus Participants. Can Med Assoc J 1996; 155: 77-88.
    [14] Pearce N, Beasley R, Crane J, et al. End of the New Zealand asthma mortality epidemic. Lancet 1995; 345: 414.
    [15] Allen & Hanbury Ltd. Personal communication, 1991.
    [16] Rubin PC. Prescribing in pregnancy. Oxford Textbook of Medicine, 3rd edn. In: Weatherall DJ, Ledingham JGG, Warrell DA, eds. Oxford: Oxford University Press, 1997; 1809-13.
    [17] Wynn M, Wynn A. The Case for Preconception Care of Men and Women. Bicester: AB Academic Publishers, 1991; 169.
    [18] Calabrese EJ. Nutritional and Environmental Health: The Influence of Nutritional Status on Pollutant Toxicity and Carcinogenicity. Vol. 1: The Vitamins, Vol. 11: Minerals and Macronutrients. New York: John Wiley, 1980; 1981.
    [19] Davies S, Downing D. Nutritional medicine. A step in the right direction? (editorial). J Nutr Med 1990; 1: 3-7.
    [20] Davies S. Nutritional flat earthers (editorial). J Nutr Med 1990; 1: 167-70.
    [21] Davies S. Nutritional medicine: has it a role in handicap prevention and the medical treatment of infertility including assisted ovulation, in vitro fertilization (IVF) and gamete intrafallopian transfer (GIFT). J Nutr Med 1990; 1: 251-8.
    [22] Fratta ID, Sigg EB, Maiorana K. Teratogenic effects of thalidomide in rabbits, rats, hamsters and mice. Toxicol Appl Pharmacol 1965; 7: 268-86 (reprinted J Nutr Med 1990; 1: 231-2).
    [23] Ault A. Thalidomide comes close to US approval (news). Lancet 1997; 350: 873.
    [24] Eaton SB, Konner M. Paleolithic nutrition--a consideration of its nature and current implications. N Engl J Med 1985; 312: 283-9.
    [25] Davies S, McLaren-Howard J, Hunnisett A, et al. Age-related decreases in chromium levels in 51,665 hair, sweat and serum samples from 40,872 patients--implications for the prevention of cardiovascular disease and type II diabetes mellitus. Metabolism-Clin & Exp 1997; 46: 469-73.
    [26] Conway GR, Pretty JN. Unwelcome Harvest--Agriculture and Pollution. London: Earthscan Publications. 1991; 645.
    [27] Jobling S, Reynolds T, White R, et al. A variety of environmentally persistent chemicals, including some phthalate plasticisers, are weakly estrogenic. Environ Health Perspect 1995; 103: 582-7.
    [28] Ginsburg J. Tackling environmental endocrine disrupters. Lancet 1996; 347: 1501-2.
    [29] Williams GC, Nesse RM. The dawn of Darwinian medicine. Quart Rev Biol 1991; 66: 1-22.
    [30] Nesse RM, Williams GC. Evolution and Healing--The New Science of Darwinian Medicine. London: Weidenfield & Nicolson, 1995, 290.
    [31] Stevens A, Price J. Evolutionary Psychiatry--A New Beginning. London: Routledge, 1996; 267.
    [32] Laurence DR, Bennett PN. Clinical Pharmacology, 5th Edn. London: Churchill Livingstone, 1980; 78.
    [33] Davies S. Bovine spongiform encephalopathy. J Nutr Med 1990, 1: 93-4.
    [34] Purdey M. Are organophosphate pesticides involved in the causation of bovine spongiform encephalopathy (BSE)? Hypothesis based upon a literature review and limited trials on BSE cattle J Nutr Med 1994, 4: 43-82.
    [35] Davies S. Are organophosphate pesticides involved in the causation of bovine spongiform encephalopathy? (editorial). J Nutr Med 1994; 4: 5-6.
    [36] Wright LF, Saylor RP, Cecere A. Occult lead intoxication in patients with gout and kidney disease. J Rheumatol 198; 11: 517-20.
    [37] Blumer W, Reich Th. Leaded gasoline--a cause of cancer. Environment International 1980; 3: 465-71.
    [38] Blumer W. Personal communication, 1984.
    [39] Werbach MR. The Nutritional Influences on Illness, A Sourcebook of Clinical Research, 2nd edn. Tarzana, CA: Third Line Press, 1993; 229-54.
    [40] Davies S, McLaren-Howard J, Hunnisett A. A case of insulin oedema treated with nutritional supplements including chromium (abstract). Presented at the Third Conference on Trace Elements in Medicine and Biology, Les Deux Alpes, France, 1991.
    [41] Werbach MR. Nutritional influences on Mental Illness, A Sourcebook of Clinical Research, 2nd edn. Tarzana, CA: Third Line Press, 1991.
    [42] Downing D, Davies S. `Allergy: conventional and alternative concepts', A critique of the Royal College of Physicians of London's Report. J Nutr Med 1992, 3: 331-49.
    [43] Brostoff J, Challacombe S, Eds. Food Allergy and Intolerance. London: Balliere and Tindall, 1987.
    [44] Anthony H, Birtwistle S, Eaton K, et al. Environmental Medicine in Clinical Practice. Southampton: BSAENM Publications, 1997.
    [45] Effective Nutritional Medicine: The Application of Nutrition to Major Health Problems. Southampton: BSAENM, 1995.
    [46] Chamberlin TC. Studies for students: the method of multiple working hypotheses. Science 1964; 146: 347-53 (reprinted in J Nutr Med 1992; 3: 159-65).
    [47] Platt JR. Strong inference. Science 1964; 146: 347-53 (reprinted in J Nutr Med 1992; 3: 167-77).
    [48] Jaffe R. Letter in response to nutritional flat earthers. J Nutr Med 1990-1: 370-1.
    [49] Lesar TS, Lomaestro BM, Pohl H. Medication-prescribing errors in a teaching hospital--a 9-year experience. Arch Int Med 1997; 157: 1569-76.
    [50] Kuhn TS, The Structure of Scientific Revolutions, 2nd edn. Chicago: University of Chicago Press, 1970.


    1997 Withholding Research Results in Academic Life Science David Blumenthal, MD, MPP; Eric G. Campbell, PhD; Melissa S. Anderson, PhD; Nancyanne Causino, EdD; Karen Seashore Louis, PhD JAMA. 1997;277:1224-1228
    Evidence From a National Survey of Faculty
    Objectives.--
    To identify the prevalence and determinants of data-withholding behaviors among academic life scientists.
    Design.--Mailed survey of 3394 life science faculty in the 50 universities that received the most funding from the National Institutes of Health in 1993.
    Participants.--A total of 2167 faculty responded to the survey, a 64% response rate.
    Outcome Measures.--Whether respondents delayed publication of their research results for more than 6 months and whether respondents refused to share research results with other university scientists in the last 3 years.
    Results.--A total of 410 respondents (19.8%) reported that publication of their research results had been delayed by more than 6 months at least once in the last 3 years to allow for patent application, to protect their scientific lead, to slow the dissemination of undesired results, to allow time to negotiate a patent, or to resolve disputes over the ownership of intellectual property. Also, 181 respondents (8.9%) reported refusing to share research results with other university scientists in the last 3 years. In multivariate analysis, participation in an academic-industry research relationship and engagement in the commercialization of university research were significantly associated with delays in publication. Odds ratios (ORs) and 95% confidence intervals (CIs) were 1.34 (1.07-1.59) and 3.15 (2.88-3.41), respectively. Variables associated with refusing to share results were conducting research similar to the Human Genome Project (OR, 2.09; 95% CI, 1.75-2.42), publication rate (OR, 1.02; 95% CI, 1.01-1.03), and engagement in commercialization of research (OR, 2.45; 95% CI, 2.08-2.82).
    Conclusions.--Withholding of research results is not a widespread phenomenon among life-science researchers. However, withholding is more common among the most productive and entrepreneurial faculty. These results also suggest that data withholding has affected a significant number of life-science faculty and further study on data-withholding practices is suggested.


    2001 Blood levels of selected hormones in patients with multiple sclerosis Elżbieta Zychwardowska, Andrzej Wajgt 1st Chair and Department of Neurology, Silesian Medical University in Katowice, Poland Med Sci Monit, 2001; 7(5): 1005-1012

    Summary:
    Background:
    Hormonal studies in patients with multiple sclerosis are rare and they often produce results which are difficult to interpret. These investigations, however, are becoming more and more important as they may cast some light on possible interrelationships between hormonal and immune systems. The aim of the present work was to investigate endocrine function in patients with multiple sclerosis on the basis of blood levels of selected pituitary (TSH, ACTH, GH) and thyroid hormones (T3, T4), and cortisol.
    Material and methods: Forty-nine MS subjects, including 25 menstruating women, 6 post-menopausal women and 18 men were included in the analysis. The hormones were measured by radioimmunoassay and immunoradiometric assay kids.
    Results: Pituitary function in respect of TSH, corticotropin and growth hormone secretion was normal. Both men and women suffering from multiple sclerosis manifested low serum T3 concentrations coexisting with normal T4 levels which may indicate changed peripheral conversion pathway of thyroid hormones. On the other hand, the disturbances in pituitary-adrenal cortex system in respect of glycocorticosteroid secretion were not observed.
    Conclusion: Normal function in respect to pituitary hormones (TSH, corticotropin, growth hormone) and normal T4 level versus low serum T3 concentration may indicate changes in peripheral conversion pathway of thyroid hormones in MS patients.


    1105
    Background:
    In the etiopathogenesis of multiple sclerosis various factors such as genetic, autoimmune and environmental ones are considered [1]. Not many studies emphasised the role of hormonal disturbances in patients with multiple sclerosis.
    Our earlier works on the concentrations of hormones in patients with multiple sclerosis showed hyperprolactinemia in men and women suffering from MS and elevated oestradiol levels in men with MS with normal testosterone concentrations.Serum levels of gonadotropic hormones (FSH and LH) in patients with MS was found normal [2].
    In recent years, it has become particularly important to investigate the nature of interrelationships between immune and neurohormonal systems [3-7]. Patients suffering from multiple sclerosis manifest abnormal humoral and cellular response to CNS antigens as well as intensive immunoglobulin synthesis and insufficient suppression mechanisms related to T cells [8-10].
    ....
    1009
    ....

    DISCUSSION
    The present paper focuses on the function of endocrine glands in patients with multiple sclerosis which was evaluated on the basis of selected pituitary, thyroid and adrenal hormones. Some MS patients manifest thyroid dysfunction [19-21].
    ....
    Thyroxin is the main hormone produced by thyroid gland, and it plays the role of prohormone.
    Thyroxin undergoes gradual deiodination. The deiodination of external ring in 5' position results in the formation of bioactive hormone - triiodothyronine (T3) which acts on the target cell through nuclear receptor. Reduced triiodothyronine levels with normal TSH and thyroxin concentrations may indicate a change in peripheral conversion of thyroid hormones in patients with multiple sclerosis.
    The most likely hypothesis accounting for this fact is the presence of low T3 syndrome, determined by impaired T4 conversion to T3 and enhanced T4 conversion to T3 reverse.
    As a result, this syndrome is characterised by low total T3 and fT3 levels and elevated rT3 concentration as well as either low, normal or even increased (rarely) fT4 levels. The concentration of TSH is normal. Low T3 syndrome occurs in numerous acute and chronic diseases, not directly related to pathologies of thyroid gland [26,27].
    ....
    The hypothesis advocating the role of autoimmune mechanisms in the development of thyroid dysfunction is also supported by the presence of anti--thyroglobulin and anti-microsomal antibodies detected in MS patients [22-24].
    ....
    The assessment of ACTH and cortisol secretion in MS subjects has been investigated by some authors, although the main focus was usually on the treatment with glycocorticosteroids [32-35].
    The results of numerous works indicate that steroids are an effective tool in the reduction of MS attacks [36-39]. However, there are controversies concerning the final outcome of steroid therapy in
    patients with multiple sclerosis.
    Adverse reactions to corticotherapy in these patients include mainly a depressive effect of pituitary - hypothalamic system as well as secondary, post--steroid adrenal cortex insufficiency.
    A distinct problem is the evaluation of hypothalamic-pituitary-adrenal axis in patients with multiple sclerosis remaining on glycocorticosteroid therapy or treated long time before the assessments. Analy-

    1010
    sed patients manifested normal blood levels of adrenocorticotropic hormone and cortisol under normal conditions (tables 4 and 5).
    ....
    Patients with MS remission usually manifested normal cortisol level, however, function tests revealed the presence of significant disturbances in hypothalamic-pituitary-adrenal axis
    .....
    Further studies are required to define the role of glycocorticosteroids in MS pathogenesis, and particularly their effect on inflammatory-demyelinisation processes in central nervous system.
    Our study showed normal pituitary function in patients with multiple sclerosis concerning the secretion of growth hormone (Table 6).
    So far, only few reports have been published on the analysis of growth hormone in patients with inflammatory-demyelinisation processes taking place in central nervous system [44,45].
    ....
    The observations on the role of growth hormone in immune system function may be of some importance for better understanding of GH role in MS etiopathogenesis.
    The results presented in this paper indicate the presence of hormonal disorders in patients with multiple sclerosis. These disturbances include mainly a change in peripheral conversion pathway of thyroid hormones. The alterations in endocrine system functions observed in subjects with multiple sclerosis may contribute to better understanding of the etiopathogenesis and clinical characteristics of this disease. The presence of hormonal disorders in MS subjects may also be an indication for therapy modification.

    CONCLUSIONS
    Low T3 levels were observed in all analysed groups of patients with multiple sclerosis. The coexistence of low T3 levels with normal T4 and TSH concentrations may be the evidence for changed peripheral conversion pathway of thyroid hormones.
    The disturbances in pituitary-adrenal cortex system in respect of glycocorticosteroid secretion were not observed in analysed patients.
    Pituitary function concerning TSH, corticotropin and growth hormone secretion was found normal.

    REFERENCES:
    1. Dijkstra CD, Polman CH, Berkenbosch F: Multiple sclerosis: some possible therapeutic opportunities. Trends Pharmacol Sci, 1993; 14: 124-129 1011
    2. Zych-Twardowska E, Wajgt A: Serum prolactin and sex hormone concentrations in patientnts with multiple sclerosis. Med Sci Monit, 1999; 5(2): 216-220
    3. Chrousos GP: The hypothalamic-pituitary-adrenal axis and immune-mediated inflammation. N Engl J Med, 1995; 332: 1351-1362
    4. Homo-Delarche F, Dardenne M: The neuroendocrine-immune axis. Springer Semin Immunopathol, 1993; 14: 221-238
    5. Johnson RW, Arkins S, Dantzer R, Kelley KW: Hormones, lymphohemopoietic
    cytokines and the neuroimmune axis. Comp Biochem Physiol A Physiol, 1997; 116: 183-201
    6. Karalis K, Muglia LJ, Bae D et al: CRH and the immune system. J Neuroimmunol, 1997; 72: 131-136
    7. Kruger TE: Immunomodulation of peripheral lymphocytes by hormones of the hypothalamus-pituitary-thyroid axis. Adv Neuroimmunol, 1996; 6: 387-395
    8. Achiron A, Mendel M, Rechavi G et al: Changes in T-cell subpopulations in multiple sclerosis. Ann Neurol, 1989; 26: 291-292
    9. Seyfert S, Klapps P, Meisel Cet al: Multiple sclerosis and other immunologic diseases. Acta Neurol Scand, 1990; 81: 37-42
    10. Verselis SJ, Goust JM: CD4+ T cell activation in multiple sclerosis. J Neuroimmunol, 1987, 14, 75-85
    11. Scherbaum WA, Schrell U, Gluck M. et al: Autoantibodies to pituitary corticotropin-producing cells: possible markers for unfavourable outcome after pituitary microsurgery for Cushing's disease. Lancet, 1987;1: 1394-1398
    12. Zoukos Y, Leonard J. P, Thomaides T et al: Beta-adrenergic receptor density and function of peripheral blood mononuclear cells are increased in multiple sclerosis: a regulatory role for cortisol and interleukin-1. Ann Neurol, 1992; 31: 657-662
    13. Hattori N, Shimatsu A, Sugita M et al: Immunoreactive growth hormone (GH) secreted by human lymphocytes: Augmented release by exogenous GH. Biochem. Biophys Res Commun, 1990; 168: 396-401
    14. Homo-Delarche F, Fitzpatrick F, Christeff N et al: Sex steroids, glucocorticoids, stress and autoimmunity. J Steroid Biochem Molec Biol, 1992; 40: 619-637
    15. Reder AT, Pinamaneni S, Smyka W, Nutter D: ACTH production by human mononuclear cells. Ann N Y Acad Sci, 1988; 540: 589-591
    16. Weigent DA, Blalock JE: Interactions between the neuroendocrine and immune systems: common hormones and receptors. Immunol Rev, 1987; 190: 79-108
    17. Durelli L, Poccardi G, Cavallo R: CD8+ high CD11b+ low T cells (T suppressor-effectors) in multiple sclerosis cerebrospinal fluid are increased during high dose corticosteroid treatment. J Neuroimmunol, 1991; 31: 221-228
    18. Pitzalis C, Sharrack B, Gray I. A et al: Comparison of the effects of oral versus intravenous methylprednisolone regimens on peripheral blood T Iymphocyte adhesion molecule expression, T cell subsets distribution and TNF alpha concentrations in multiple sclerosis. J Neuroimmunol,
    1997; 74: 62-68
    19. Iwasaki Y, Kinoshita M: Thyroid function in patients with multiple sclerosis. Acta Neurol Scand, 1988; 77: 269
    20. Kiessling WR, Pflughaupt KW, Haubitz I, Mertens HG: Thyroid function in multiple sclerosis. Acta Neurol Scand, 1980; 62: 255-258
    21. Klapps P, Seyfert S, Fischer T, Scherbaum WA: Endocrine function in multiple sclerosis. Acta Neurol Scand, 1992; 85: 353-357
    22. De Keyser J: Autoimmunity in multiple sclerosis. Neurology, 1988; 38: 371-374
    23. Ioppoli C, Meucci G, Mariotti S et al: Circulating thyroid and gastric parietal cell autoantibodies in patients with multiple sclerosis. Ital J Neurol Sci, 1990; 11: 31-36
    24. Kiessling WR, Pflughaupt KW: Antithyroid antibodies in multiple sclerosis.
    Lancet, 1980; 1: 41
    25. Poser C. M, Paty D. W, Scheinberg L et al: New diagnostic criteria for multiple sclerosis: guideline for research protocols. Ann Neurol, 1983;13: 227-231
    26. Wartofsky L, Burman K: Alterations in thyroid function in patients with systemic illness: the 'euthyroid sick syndrome'. Endoc Rev, 1982;3: 164-216
    27. Chopra I, Chopra U, Reza M et al: Reciprocal cheges in serum concentrations of 3, 3', 5' trijodothyronine (T3) in systemic illness. J Clin Endocrinol. Metab, 1975; 41: 1043-1049
    28. Kira J, Harada M, Yamaguchi Y et al: Hyperprolactinemia in multiple sclerosis. J. Neurol. Sci, 1991, 102, 61-66.
    29. McCombe P. A, Chalk J. B, Pender M. P. : Familial occurence of multiple sclerosis with thyroid disease and systemic lupus erythematosus. J Neurol Sci, 1990; 97: 163-171
    30. Roquer J, Esccudero D, Herraiz J et al: Multiple sclerosis and Hashimoto's thyroiditis. J Neurol, 1987; 234: 23-24
    31. Hinterberger-Fischer M, Kier P, Forstinger I et al: Coincidence of severe aplastic anaemia with multiple sclerosis or thyroid disorders. Report of 5 cases. Acta Haematol, 1994; 92: 136-139
    32. Levic Z, Micic D, Nicolic J et al: Short-term high dose steroid therapy does not affect the hypothalamic-pituitary-adrenal axis in relapsing multiple sclerosis patients. Clinical assessment by the insulin tolerance test. J Endocrinol Invest, 1996; 19: 30-34
    33. Miro J, Amado J. A, Pesquera Cet al: Assessment of the hypothalamic-pituitary-adrenal axis function after corticosteroid therapy for MS relapses. Acta Neurol Scand, 1990; 81: 524-528
    34. Polman CH, van der Wiel HE: Adrenal function after corticosteroid treatment in MS. Acta Neurol Scand, 1991; 83: 205-207
    35. Wenning GK, Wietholter H, Schnauder G et al: Recovery of the hypothalamic-pituitary-adrenal axis from suppression by short-term, high--dose intravenous prednisolone therapy in patients with MS. Acta Neurol Scand, 1994; 89: 270-273
    36. Barnes D, Hughes RA, Morris RW et al: Randomised trial of oral and intravenous methylprednisolone in acute relapses of multiple sclerosis. Lancet, 1997; 349: 902-906
    37. Cazzato G, Mesiano T, Antonello R et al: Double-blind, placebo-controlled, randomized, crossover trial of high-dose methylprednisolone in patients with chronic progressive form of multiple sclerosis. Eur Neurol, 1995; 35: 193-198
    38. Franciotta D, Picollo G, Zardini E et al: Soluble CD8 and ICAM-1 in serum and CSF of MS patients treated with 6-methylprednisolone. Acta Neurol Scand, 1997; 95: 275-279
    39. Francis DA: The current therapy of multiple sclerosis. J Clin Pharm Ther, 1993; 18: 77-84
    40. Grasser A, Moller A, Backmund H et al: Heterogeneity of hypothalamic--pituitary-adrenal system response to a combined dexamethasone-CRH test in multiple sclerosis. Exp Clin Endocrinol Diabetes, 1996; 104: 31-37 Clinical Research
    41. Nowak S, Kowalski D, Kowalska K, Banasiƒska E: ACTH w osoczu i p.ynie mózgowo-rdzeniowym chorych na stwardnienie rozsiane. Neurol Neurochir Pol, 1986; 20: 542-546
    42. Reder AT, Lowy MT, Meltzer HY, Antel JP: Dexamethasone suppression test abnormalities in multiple sclerosis: relation to ACTH therapy.Neurology, 1987; 37: 849-853
    43. Michelson D, Stone L, Galliven E et al: Multiple sclerosis is associated with alterations in hypothalamic-pituitary-adrenal axis function.J Clin Endocrinol Metab, 1994; 79: 848-853
    44. Fischer K. : Multiple sclerosis and the evolution of growth hormone mechanisms in man. Med Hypotheses, 1988; 27: 99-106
    45. Nowak S, Kowalski D, Kowalska K et al: Hormon wzrostu w surowicy krwi i p.ynie mózgowo-rdzeniowym chorych na stwardnienie rozsiane.Neurol Neurochir Pol, 1987; 21: 315-318
    46. Moller A, Hansen BL, HansenGN, Hagen C: Autoantibodies in sera from patients with multiple sclerosis directed against antigenic determinants in pituitary growth hormone-secreting cells and in structures containing vasopressin/oxytocin. J Neuroimmunol, 1985; 8: 177-184
    47. Davies JS, Hinds NP, Scanlon MF: Growth hormone deficiency and hypogonadism in a patient with multiple sclerosis. Clin Endocrinol Oxf, 1996; 44: 117-119
    48. Geffner ME, Bersch N, Lippe BM et al: Growth hormone mediates the growth of T-lymphoblast cell lines via locally generated insulin-like growth factor-1. J Clin Endocrinol Metab, 1990; 71: 464-469
    49. Rapaport R, Oleske J, Ahdieh H et al: Effects of human growth hormone on immune functions: In vitro studies on cells of normal and growth hormone-deficient children. Life Sci, 1987; 41: 2319-2324
    50. Kelley KW: Growth hormone, lymphocytes and macrophages. Biochem Pharmacol, 1989; 38: 705-713
    51. Goff BL, Roth JA, Arp LH, Incefy GS: Growth hormone treatment stimulates thymulin production in aged dogs. Clin Exp Immunol, 1987; 68: 580-587

    Reporting Financial Conflicts of Interest and Relationships Between Investigators and Research Sponsors Editorial JAMA Vol. 286 No. 1, July 4, 2001

    Catherine D. DeAngelis, MD, MPH; Phil B. Fontanarosa, MD; Annette Flanagin, RN, MA

    The financial aspects of biomedical research currently are under intense examination. Increasing attention and concern have been directed toward the financial ties of individual investigators as well as the complex relationships among researchers, academic medical centers, commercial clinical research entities, and industry.
    Medical journal editors are responsible for evaluating the scientific validity and credibility of research submitted for possible publication. Editors also have an obligation to present pertinent information related to the financial aspects of the articles they publish so readers can interpret the findings in light of this information. THE JOURNAL has policies governing financial aspects of manuscripts submitted, reviewed, edited, and published. Herein, we describe a more specific policy for reporting authors' financial conflicts of interest and a new policy for reporting relationships between investigators and research sponsors.
    ....
    Since 1985, THE JOURNAL has requested authors to disclose financial interests related to the subject matter of their research and since 1989 has required authors to submit signed financial disclosure statements. In the years that have followed, and because of increased concern about conflicts of interest, we have made our policies for reporting financial interests more stringent and more specific.
    ....
    In addition to requiring information on financial interests from authors, THE JOURNAL has, since 1987, routinely requested all peer reviewers who complete a manuscript review to disclose any potential conflicts of interest, financial or otherwise, they may have related to that manuscript. Such information is kept confidential and is not revealed to the authors of the manuscript or to other reviewers. Peer reviewers who believe they have a conflict of interest (financial or otherwise) that prevents them from providing an objective review are instructed to disqualify themselves from reviewing that paper and to return the manuscript without completing a review.
    ....
    Another important and increasingly prevalent aspect of medical research that can lead to conflicts of interest involves the relationships between scientific investigators and industry and the direct involvement of study sponsors in the research. Industry invested approximately $55 to $60 billion in research and development in 2000 (compared with $25 billion in US federal spending on research), and industry also provides an estimated 70% of funding for clinical drug trials in the United States. Without industry-sponsored research, some important advances and discoveries in medical research might not occur.
    However, active involvement in and control of research investigations (such as control of data, performance of statistical analyses, complete authority over manuscript preparation and decisions to submit for publication) by companies whose products are being evaluated and who have a vested financial interest in the study outcome represents a clear conflict of interest. Moreover, this level of involvement and control of the research could be viewed as the sponsor having the potential to influence the study results and might create doubts about the validity of the research. These concerns are not without foundation; previous reports have documented several major problems in some industry-sponsored studies, including issues related to trial design, data availability, and control over publication.
    ....

    As another mechanism to help assure complete reporting of study outcomes, the editors may request and review the original study protocol for any research investigation. These approaches should help convince readers about the integrity of the data and analyses presented, and should help eliminate uncertainty that some readers might have because of the sponsor's involvement in the research.
    In an ideal world, physicians, patients, and the public would not have to be concerned about conflicts of interest related to medical research or have questions about the role of sponsors in industry-funded research. However, to respond to these current real-world concerns, THE JOURNAL will require clear reporting of authors' financial conflicts of interest and clear description of the involvement of sponsors in medical research. Even though we recognize that these efforts are not fail-safe, we hope that such reporting will help to ensure the integrity of medical science, enable readers to interpret the results of scientific studies appropriately, and maintain public confidence in biomedical research.


    REFERENCES
    Moses H, Martin JB. Academic relationships with industry: a new model for biomedical research. JAMA.
    2001;285:933-935.
    Boyd EA, Bero LA. Assessing faculty financial relationships with industry: a case study. JAMA. 2000;284:2209-2214.
    DeAngelis CD. Conflict of interest and the public trust. JAMA. 2000;284:2237-2238. Stelfox HT, Chua G, O'Rouke K, Detsky AS. Conflict of interest in the debate over calcium channel antagonists. N Engl J Med. 1998;338:101-106.
    Angell M. Is academic medicine for sale? N Engl J Med. 2000;342:1516-1518.
    Bodenheimer T. Uneasy alliance: clinical investigators and the pharmaceutical industry. N Engl J Med. 2000;342:1539-1544.
    Martin JB, Kasper DL. In whose best interest? breaching the academic-industry wall. N Engl J Med.2000;343:1646-1649.
    Krimsky S, Rothenberg LS. Conflict of interest policies in science and medical journals: editorial practices and author disclosures. Sci Eng Ethics. 2001;7:205-218.
    Flanagin A. Ethical and legal considerations. In: Iverson CI, Flanagin A, Fontanarosa PB, et al. American Medical Association Manual of Style: A Guide for Authors and Editors. 9th ed. Philadelphia, Pa: Williams & Wilkins; 1998:87-172.
    Korn D. Conflicts of interest in biomedical research. JAMA. 2000;284:2234-2237.
    Friedman PJ. The troublesome semantics of conflict of interest. Ethics Behav. 1992;2:245-251.
    Flanagin A. Conflict of interest. In: Jones AH, McClellan F, eds. Ethical Issues in Biomedical Publication Baltimore, Md. Johns Hopkins Press; 2000:137-165.
    Guidelines for dealing with faculty conflicts of commitment and conflicts of interest in research. 1990. Available at: http://www.aamc.org/research/dbr/coi.htm
    Cohen JJ. Trust us to make a difference: ensuring public confidence in the integrity of clinical research. Acad Med. 2001;76:209-214.
    Constantian MB. Conflicts of interest in medical writing and the concept of disclosure. Plast Reconstr Surg. 2000;105:796-797.
    Knoll E, Lundberg GD. New instructions for JAMA authors. JAMA. 1985;254:97-98.
    Lundberg GD, Flanagin A. New requirements for authors: signed statements of authorship responsibility and financial disclosure. JAMA 1989;262:2003-2004.
    Instructions for Authors. JAMA. 2001;286:101-108.
    Cho MK, Shohara R, Schissel A, Rennie D. Policies on faculty conflicts of interest at US universities. JAMA. 2000;284:2203-2208.
    Lo B, Wolf LE, Berkeley A. Conflict-of-interest policies for investigators in clinical trials. N Engl J Med. 2000;343:1616-1620.
    McCrary SV, Anderson CB, Jakovljevic J, et al. A national survey of policies on disclosure of conflicts of interest in biomedical research. N Engl J Med. 2000;343:1621-1626.
    Cech TR, Leonard JS. Conflicts of interest: moving beyond disclosure. Science. 2001;291:989.
    Southgate MT. Conflict of interest and the peer review process. JAMA. 1987;258:1375.
    Blumenthal D, Causino N, Campbell E, Louis KS. Relationships between academic institutions and industry in the life sciences: an industry survey. N Engl J Med. 1996;334:368-373.
    Rennie D. Thyroid storm. JAMA. 1997;277:1238-1243.
    International Committee of Medical Journal Editors. Uniform requirements for manuscripts submitted to biomedical journals. Updated May 2000. Available at: http://www.icmje.org
    Rennie D, Flanagin A, Yank V. The contributions of authors. JAMA. 2000;284:89-90.

    2001 Letter in response to Colin Dayan's article ' Interpretation of thyroid function tests'. Lancet 2001; 357: 619-24. Dr PBS Fowler The Lancet, Volume 357, Number 9273 23 June 2001
    ....
    Probably, thyroid function tests are done at least 50 times more commonly to assess thyroxine dose than to diagnose thyroid dysfunction.
    ....
    Every qualifying medical student knows that thyrotoxicosis can cause atrial fibrillation or osteoporosis and makes the illogical extrapolation that a patient given a dose of thyroxine that suppresses or brings the serum thyroxine concentration to higher than the reference range is receiving too much replacement. It is excusable for doctors to believe that if the serum thyroxine concentration is higher than the so-called normal range that the dose of thyroxine should be decreased. In fact, the thyroxine is replacing the thyroxine and tri-iodothyronine, which is produced by a normal gland. The only function of doing a thyroxine estimation is to confirm that the patient is taking the thyroxine and that it is being absorbed. Serum thyroxine concentration, if raised, confirms that the thyroxine replacement dose is inadequate but a suppressed concentration does not necessarily show that the dose is too high. Serum T3 must be kept within the normal range.
    Before the days of hormone assays, hypothyroid patients received about double the average dose of thyroxine given today, but did not develop osteoporosis or atrial fibrillation. Doses should be judged clinically rather than be governed by misinterpreted hormone results.
    P B S Fowler

    1 Dayan CM. Interpretation of thyroid function tests. Lancet 2001; 357: 619-24.

    2001 Thyroxine treatment in patients with symptoms of hypothyroidism but thyroid function tests within the reference range: randomised double blind placebo controlled crossover trial M Anne Pollock, principal biochemist a, Alison Sturrock, senior house officer b , Karen Marshall, trainee clinical psychologist c, Kate M Davidson, research tutor c, Christopher J G Kelly, Specialist registrar b, Alex D McMahon, consultant statistician and E Hamish McLaren, consultant physician b BMJ 2001;323:891-895 (20 October)
    a Department of Clinical Biochemistry, Stobhill Hospital, Glasgow G21 3UW, b Department of Medicine, Stobhill Hospital, c Department of Psychological Medicine, Gartnavel Royal Hospital, Glasgow G12 0XH, d Robertson Centre for Biostatistics, University of Glasgow, Glasgow G12 8QQ
    .....
    Introduction
    The classic symptoms of hypothyroidism are wide ranging and non-specific, therefore biochemical testing has become the cornerstone of diagnosis in patients for whom there is a clinical suspicion of thyroid dysfunction. However, recent anecdotal evidence has suggested there may be some clinical benefit in giving thyroxine to patients with symptoms of hypothyroidism who have thyroid function tests within the reference range. 1-3 After a series of reports in our local newspaper suggesting that such patients benefited from thyroxine therapy we treated two patients empirically with thyroxine, and they both reported symptomatic relief. 4
    To investigate this further, we conducted a double blind placebo controlled crossover trial of thyroxine in patients who had symptoms of hypothyroidism but whose thyroid function tests were within the reference range. A group of controls, who were similar in age and sex to the patient group, took part in a parallel trial. The same protocol was used for controls and patients to test the clinical belief that thyroxine treatment would have an effect on wellbeing even in participants without symptoms of hypothyroidism. We assessed response to thyroxine by using a battery of biochemical, physical, and psychological tests.
    .....
    Discussion
    This is the first randomised double blind placebo controlled trial of thyroxine treatment in patients who have symptoms of hypothyroidism but are biochemically euthyroid
    .....
    Controls showed no significant changes in psychological measurements after treatment with either thyroxine or placebo. This suggests that, contrary to widespread belief, thyroxine does not have a non-specific effect on wellbeing. In the participants who received placebo first, patients showed a small but significant improvement in general health, physical wellbeing, and anxiety and depression after placebo when compared with baseline. Thyroxine treatment, however, had no greater effect than placebo in this group of patients. This contrasts with previous studies in biochemically hypothyroid patients, where thyroxine treatment was associated with psychological improvement.
    ....
    Conclusion
    We can find no support for the hypothesis that people with symptoms of hypothyroidism but thyroid function tests within the reference range benefit from treatment with 100 ?g thyroxine daily. However, our results require confirmation in a larger study. The improvement noted anecdotally and in open studies may be due to the placebo effect shown in our study.

    Referencees
    1. Skinner, GRB., Thomas, R., Taylor, M., Sellarajah, M., Bolt, S., & Krett, S. Thyroxine should be tried in clinically hypothyroid, but biochemically euthyroid patients. BMJ 1997; 314: 1764.
    2. Williams, G. Distinguishing hypothyroid symptoms from common non-specific complaints is difficult. BMJ 1997; 315: 814.
    3. Holmes, Diana, . Tears behind closed doors. London: Avon, 1998.
    4. Mclaren, EH., Kelly, CJG., & Pollock, MA. Trial of thyroxine treatment for biochemically euthyroid patients has been approved. BMJ 1997; 315: 1463.
    5. Wechsler, D. Wechsler memory scale-revised manual. San Antonio: Psychological Corporation, 1987.
    6. Reitan, RM. A research programme on the psychological effects of brain lesions in human beings. In: Ellis, NR., ed. International review of research in mental retardation. New York: Elsevier, 1966.
    7. Zigmund, AS. & Snaith, RP. The hospital anxiety and depression scale. Acta Psychiatr Scand 1983; 67: 361370.
    8. Ware, JE. SF36 health survey: manual and interpretation guide. Health Institute: New England Medical Centre, 1997.
    9. Skinner, GRB., Holmes, D., Ahmad, A., Davies, JA., & Benitez, J. Clinical response to thyroxine sodium in clinically hypothyroid but biochemically euthyroid patients. J Nutr Environ Med 2000; 10: 115124.
    10. Beckwith, BC. & Tucker, DM. Thyroid disorders. In: Tarter, RE., Van Thiel, DH., & Edwards, KL., eds. Medical neuropsychology: the impact of disease on behaviour. New York: Plenum Press, 1998.
    11. Denicoff, KD., Joffe, RT., Lakshmanan, MC., Robbins, J., & Rubinow, DR. Neuropsychiatric manifestations of altered thyroid state. Am J Psychiatr. 1990; 147: 94
    12. Osterweil, D., Syndulko, K., & Cohen, SN. Cognitive function in non-demented older adults with hypothyroidism. J Am Geriatr Soc 1992; 40:335


    treating thyroid deficiency updated with:
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    1998 Bone Changes in Pre- and Postmenopausal Women with Thyroid Cancer on Levothyroxine Therapy: Evolution of Axial and Appendicular Bone Mass E. Jodar (*), M. Begona Lopez (*), L. Garcia, D. Rigopoulou, G. Martinez, F. Hawkins(1) Service of Endocrinology, University Hospital 12 de Octubre, Madrid, Spain Osteoporosis International Volume 8 Issue 4 (1998) pp 311-316

    Abstract: The effects of suppressive doses of levothyroxine (LT 4 on bone mass are controversial. Our aim was to evaluate the effects on axial and appendicular bone mineral density (BMD) and bone metabolism of long-term LT4 suppressive therapy in women by means of cross-sectional and longitudinal studies, and also to assess the potential influence of menopausal status and LT4 dose. Seventy-six women (aged 47 + 13 years, 37 pre- and 39 postmenopausal) on suppressive therapy (67 + 34 months duration, mean LT4 dose 168 + 41 mg/day) from our Thyroid Cancer Unit without previous hyperthyroidism or concomitant hypoparathyroidism were studied. Serum TSH, T3 free T4, calcium, phosphorus, alkaline phosphatase, BGP, iPTH and urinary calcium (uCA) were measured. BMD was measured by dual-energy X-ray absorptiometry (DXA) at lumbar spine, femoral neck, Ward's triangle, ultradistal and distal third radius and expressed as a Z-score. In a subset of 27 women aged 46 + 15 years (14 pre- and 13 postmenopausal) a second densitometry scan was performed 27 + 5 months later. Patients on suppressive therapy showed a small reduction in BMD at the distal third radius (Z-score: 70.77 + 0.98; 95% confidence interval: 71.11, 70.44) without differences between pre- and postmenopausal women. Significant relations with the regimen of suppressive therapy and bone turnover markers were detected except at the lumbar spine. In the longitudinal study a significant although mild reduction in femoral neck BMD was found that correlated with prior T3 and iPTH. In conclusion, our data show a small detrimental effect of cautious LT4 suppressive therapy on bone mass assessed by DXA; it remains to be established whether this increases the prevalence of fractures.


    Thyroid cancer (*) The contributions of Esteban Jodar and Maria Begona Lopez are equal and the order of authorship is arbitrary. (*) The contributions of Esteban Jodar and Maria Begona Lopez are equal and the order of authorship is arbitrary.

    1999 Syndromes of resistance to thyroid hormone: Clinical considerations Vlaeminck-Guillem, V.; Wemeau, J.L. Revue de Medecine Interne, Vol: 20, Issue: 12, 1114-1122, 1999
    Abstract (English):

    Introduction. - Syndromes of resistance to thyroid hormone correspond to variable clinical states which are usually transmitted as autosomal dominant traits and characterized by the lack of sensitivity of target tissues to triiodothyronine (T3). The diagnosis has to be performed in order to offer an appropriate therapy.
    Current knowledge and key points. - Clinical states range between two extremes: the generalized form, with global euthyroidism, and the predominantly pituitary form, with thyrotoxicosis. Surprisingly, these various clinical situations are usually determined by the same genetic defect, i.e., an anomaly of one of the two alleles of the gene encoding the thyroid hormone receptor TRâ. High levels of circulating thyroid hormones in the presence of detectable thyroid stimulating hormone (TSH) levels is the characteristic biological feature. Pituitary thyreotropic adenoma, another etiology of inappropriate secretion of TSH, needs thus to be ruled out. No treatment is required in case of generalized resistance to thyroid hormone, whereas two specific drugs (TRIAC and D-T4) appear to be useful in the predominantly pituitary form.
    Future prospects and projects. - Mechanisms of resistance have been well documented, therefore allowing better understanding of T3 action on its nuclear receptor. Several transcriptional cofactors or corepressors have been identified and have to be investigated to explain the intriguing inter- and intra-familial, and even intra-individual, phenotypic variability. New insights should, furthermore, be gained from these studies to precisely determine how therapeutic agents work in resistance to thyroid hormone.

    2001 AACE Press release: January 18, 2001
    New Campaign Urges People to "Think Thyroid" at Critical Life Stages and Get Tested
    JACKSONVILLE, FL, January 18, 2001

    January is Thyroid Awareness Month
    New York, NY - January 18, 2001 - Fewer than fifteen percent of Americans correctly identified the post childbirth (postpartum) period, menopause, or over 60 years of age, as key life stages when thyroid disease often strikes, according to a national survey released today by the American Association of Clinical Endocrinologists (AACE). To combat this lack of awareness, AACE is launching a new campaign, "The Neck's Time is Now," to educate Americans about the pivotal times, from birth to advanced age, when people are at increased risk for developing a thyroid disorder. Americans need to "think thyroid" and see their doctor for a TSH (thyroid stimulating hormone) blood test when: pregnant women go for their first prenatal visit; following pregnancy if postpartum depression strikes; a child's growth or behavior patterns change; mood swings and other symptoms of menopause persist despite hormone replacement therapy; fatigue, depression and forgetfulness plague older Americans. The thyroid is a butterfly-shaped gland located in the neck, just below the Adam's apple and above the collarbone. Left untreated, thyroid disease causes serious long-term complications such as elevated cholesterol levels and subsequent heart disease, infertility, muscle weakness and osteoporosis. Thyroid disease affects more than 13 million Americans, yet more than half remain undiagnosed.
    "The millions who remain undiagnosed reflect the widespread lack of awareness of this serious, but easily treatable condition. While more than half of the respondents can tell you their blood pressure, and more than one in five can identify their cholesterol (39%) and glucose levels (21%), only fifteen percent know their thyroid function - even though the thyroid gland influences these levels, according to the AACE survey. "For the millions of Americans affected by thyroid disease, it is important that they learn to recognize and evaluate the subtle signs and symptoms that can be significant markers of thyroid disease for themselves or for a loved one," says Paul Jellinger, M.D., F.A.C.E., President of AACE and Clinical Professor at the University of Miami School of Medicine.
    Detecting and Understanding TSH
    "Despite the critical need for detecting thyroid disorders early to avoid serious complications, the survey also revealed that almost 60 percent of Americans have never been tested for a thyroid condition. An overwhelming majority of survey participants (85 percent) failed to know the most common, gold standard measure of thyroid function - the TSH test. The TSH is a simple yet highly sensitive blood test that enables physicians to detect even slight abnormalities in thyroid function. It determines the level of thyroid stimulating hormone which regulates thyroid hormone production, indicating whether the thyroid gland is overactive (hyperthyroid), underactive (hypothyroid) or normal (euthyroid).
    "AACE encourages patients whose TSH is outside the normal range (.5-5.0 uU/ml) to see an endocrinologist for treatment and thyroid disease management. Even though a TSH level between 3.0 and 5.0 uU/ml is in the normal range, it should be considered suspect since it may signal a case of evolving thyroid underactivity. The new thyroid stimulating hormone test is sensitive enough to detect both hypothyroid and hyperthyroid conditions. "TSH tests play a vital role in helping physicians diagnose and manage thyroid disorders," says Hossein Gharib, M.D., F.A.C.E, a Vice-President of AACE and Professor of Medicine at the Mayo Medical School. "Constant monitoring of a patient's TSH level is critical in early detection and treatment of thyroid disease."
    The Neck's Time is Now: Thyroid Through the Ages
    There are several principal life stages at which the risk for developing a thyroid disorder increases. Because of the advanced prevalence of thyroid disease, AACE advises TSH testing during the following times:
    Birth through Adolescence: Effects on Mental and Physical Growth
    One out of every four to five thousand babies born in the U.S. has hypothyroidism. Fortunately, screening for hypothyroidism is done routinely in North America on all newborns by administering a heelpad test to uncover cretinism, a growth and mental disorder brought on by a lack of thyroid hormone.
    Parents need to be aware that thyroid disorders may also appear later in their child's development. A change in a child's growth rate is sometimes the only evidence that thyroid trouble is present because children are less likely to complain of feeling sick or to ask for help. Hyperthyroid children will rapidly outgrow new clothes, while hypothyroid children may mysteriously stop growing. But difficulty concentrating and inattentiveness in school, unexplained change in grades, hyperactivity, or unexplained daytime fatigue, may all be symptoms of an underlying thyroid condition. Children who come from families with a history of thyroid disease are especially likely to develop thyroid disorders.
    The Reproductive Years: Effects on Pregnancy
    Women who are unable to conceive should have their thyroid function assessed since thyroid disorders can impair fertility. In addition, recent studies have shown that untreated thyroid disease during pregnancy may negatively impact a child's psychological development, resulting in a lower I.Q. score and a decrease in motor skills, attention, language and reading abilities. Other studies suggest that pregnant women with hypothyroidism have a four-times greater risk for miscarriage during the second trimester. In fact, six out of every 100 miscarriages may be associated with autoimmune thyroid disease during pregnancy. AACE advises expectant mothers to take a TSH test before pregnancy or as part of the standard prenatal blood work.
    The symptoms of thyroid illness are often vague and hard to recognize, especially when they are present after a woman gives birth. In many cases, the symptoms are mistaken for other conditions such as depression. In reality, many new mothers who are diagnosed with postpartum depression may actually be suffering from a common but seldom diagnosed thyroid disorder known as postpartum thyroiditis. During this time, women may suffer from an increased heart rate, insomnia, anxiety, as well as depression. This condition usually occurs during the first few weeks after the baby is born, and can continue for up to a year. A TSH test will pinpoint postpartum thyroiditis and medication will return thyroid function to normal, and often reverses the depression.
    Midlife: Menopause Doesn't Have to Mean "Pause"
    One in three women over the age of forty still experience the common symptoms of menopause despite treatment with hormone replacement therapy (HRT), according to AACE data. In fact, common menopausal symptoms - mood swings, depression, sleep disturbances, fatigue, forgetfulness, weight gain, change in hair, skin, and nails - could actually be signs of an underlying thyroid condition. AACE recommends that all women over forty have a TSH test since studies have shown that 10 percent of women in this age group have undiagnosed thyroid disease.
    The Senior Years: Aging Without Feeling Aged
    For some older people, the golden years of life are not what they expected, due to the onset of symptoms such as fatigue, depression, forgetfulness, insomnia, and changes in appetite and weight. Most seniors erroneously assume that these feelings are a natural part of aging, when in fact these may be signs of an underlying thyroid condition. Seniors who report these symptoms to their doctors may be misdiagnosed with depression or even mild dementia. AACE underscores that aging, in the absence of disease, should not automatically be associated with the above symptoms. Since incidence of thyroid disease increases with age, and almost 20 percent of women over the age of sixty have some form of thyroid disease, TSH testing is particularly important for this age group.
    The American Association of Clinical Endocrinologists and the Neck CheckTM:
    While the TSH blood test is the most sensitive and accurate diagnostic tool, as a first step, AACE recommends that patients perform a simple self-examination called the Thyroid Neck CheckTM. This self-exam, unveiled by AACE in 1997, will help Americans detect an enlarged thyroid gland. If a patient finds an abnormality, they should speak with a physician about getting tested and treated for thyroid disease.
    The American Association of Clinical Endocrinologists (AACE) was established in 1991 and is the country's largest professional organization of clinical endocrinologists. Its membership consists of more than 3,500 clinical endocrinologists devoted to providing care for patients with endocrine disorders. The association strives to improve the public's understanding and awareness of endocrine diseases and the added value of the clinical endocrinologist in the diagnosis and treatment of these diseases.


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